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ELISA

Overview of attention for book
Cover of 'ELISA'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 The Biochemical Properties of Antibodies and Their Fragments
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    Chapter 2 Hybridoma Technology
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    Chapter 3 Affinity Purification of Antibodies
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    Chapter 4 Bioconjugation of Antibodies to Horseradish Peroxidase (HRP)
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    Chapter 5 Indirect ELISA
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    Chapter 6 Direct ELISA
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    Chapter 7 A Double-Sandwich ELISA for Identification of Monoclonal Antibodies Suitable for Sandwich Immunoassays
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    Chapter 8 ELISpot and DC-ELISpot Assay to Measure Frequency of Antigen-Specific IFNγ-Secreting Cells.
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    Chapter 9 The Western Blot
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    Chapter 10 ELISA
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    Chapter 11 Multiplexed Microsphere Suspension Array-Based Immunoassays
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    Chapter 12 Multiplex Immunoassay: A Planar Array on a Chip Using the MagArray(™) Technology.
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    Chapter 13 Lateral Flow Immunoassay
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    Chapter 14 Immuno-PCR Assay for Sensitive Detection of Proteins in Real Time
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    Chapter 15 ELISA
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    Chapter 16 Tyramide Signal Amplification for Immunofluorescent Enhancement
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    Chapter 17 Correlative Microscopy for Localization of Proteins In Situ: Pre-embedding Immuno-Electron Microscopy Using FluoroNanogold, Gold Enhancement, and Low-Temperature Resin
  19. Altmetric Badge
    Chapter 18 Multiplex ELISA Using Oligonucleotide Tethered Antibodies.
  20. Altmetric Badge
    Chapter 19 Gas Plasma Surface Chemistry for Biological Assays
Attention for Chapter 10: ELISA
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Chapter title
ELISA
Chapter number 10
Book title
ELISA
Published in
Methods in molecular biology, January 2015
DOI 10.1007/978-1-4939-2742-5_10
Pubmed ID
26160568
Book ISBNs
978-1-4939-2741-8, 978-1-4939-2742-5
Authors

Chennuru, Sreedevi, Pavuluri, Panduranga Rao, Sreedevi Chennuru, Panduranga Rao Pavuluri

Abstract

Flow-through assay (FTA) is a rapid, simple-to-perform, cost-effective, and user-friendly diagnostic test for monitoring infections in non-laboratory settings. It is mostly applied for antibody detection. FTA employing protein-A colloidal gold conjugate to detect antibodies against porcine cysticerci using cyst fluid and whole cyst antigens of Taenia solium metacestode is described here. Antibodies in the serum are captured by an antigen spotted onto a nitrocellulose membrane mounted on a flow-through device that serves as the antigen capture matrix. The bound antibodies are visualized by the addition of protein-A colloidal gold conjugate, which imparts a pink color. The test can be completed within 3 min at room temperature without any instrumentation. The sensitivity and specificity of the FTA are in agreement with ELISA.

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X Demographics

X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 14 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 14 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 3 21%
Student > Ph. D. Student 3 21%
Researcher 2 14%
Student > Master 1 7%
Other 1 7%
Other 2 14%
Unknown 2 14%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 3 21%
Veterinary Science and Veterinary Medicine 2 14%
Medicine and Dentistry 2 14%
Agricultural and Biological Sciences 1 7%
Pharmacology, Toxicology and Pharmaceutical Science 1 7%
Other 2 14%
Unknown 3 21%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 06 April 2016.
All research outputs
#20,318,358
of 22,860,626 outputs
Outputs from Methods in molecular biology
#9,916
of 13,127 outputs
Outputs of similar age
#295,996
of 353,273 outputs
Outputs of similar age from Methods in molecular biology
#636
of 997 outputs
Altmetric has tracked 22,860,626 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,127 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 353,273 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 997 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.

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