| Chapter title |
Intracytoplasmic Transport of Hepatitis B Virus Capsids
|
|---|---|
| Chapter number | 4 |
| Book title |
Hepatitis B Virus
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6700-1_4 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6698-1, 978-1-4939-6700-1
|
| Authors |
Quentin Osseman, Michael Kann |
| Abstract |
The early steps of HBV entry remain largely unknown despite the recent discovery of an HBV-specific entry receptor. Following entry HBV capsids have to be transported through the cytoplasm to the nuclear periphery, followed by nuclear entry. These steps have to take place in a coordinated manner to allow delivery of the genome into the nucleus. Due to the viscosity of the cytoplasm, the intracytoplasmic translocation has to be active and directed.Here, we describe protocols that can be applied to investigations of the HBV capsid with the cytoplasmic transport systems. We have chosen to present two independent experimental approaches, which allow avoiding artifacts. Aside of the specific capsid detection system, the protocols can be applied to any other viral structure. |
X Demographics
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|---|---|---|
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|---|---|---|
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Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
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Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Doctoral Student | 1 | 25% |
| Unknown | 3 | 75% |
| Readers by discipline | Count | As % |
|---|---|---|
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| Unknown | 3 | 75% |