Chapter title |
Detection of Hepatitis B Virus Particles Released from Cultured Cells by Particle Gel Assay
|
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Chapter number | 15 |
Book title |
Hepatitis B Virus
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6700-1_15 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6698-1, 978-1-4939-6700-1
|
Authors |
Ran Yan, Dawei Cai, Yuanjie Liu, Haitao Guo |
Abstract |
The culture fluid of HBV replicating cells contains a mixture of viral particles with different structural and genetic components, including enveloped infectious virions, genome-free virion, envelope-only subviral particles, and nonenveloped naked capsids. Based on their different physical and chemical properties, the enveloped and nonenveloped particles can be separated by the native agarose gel electrophoresis and transferred onto a positively charged microporous membrane, then the virus particle-associated protein components and nucleic acid content can be detected by antibody-based enzyme immunoassay (EIA) and hybridization, respectively. Such convenient experimental procedure is called HBV particle assay and described in detail in this chapter. The particle gel assay can be used to study viral and host regulations of HBV virus morphogenesis and egress, and for antiviral assessment of HBV inhibitors as well. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 12 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 5 | 42% |
Other | 3 | 25% |
Researcher | 1 | 8% |
Student > Postgraduate | 1 | 8% |
Unknown | 2 | 17% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 4 | 33% |
Immunology and Microbiology | 2 | 17% |
Agricultural and Biological Sciences | 2 | 17% |
Chemical Engineering | 1 | 8% |
Unknown | 3 | 25% |