Chapter title |
Advanced Method for Isolation of Mouse Hepatocytes, Liver Sinusoidal Endothelial Cells, and Kupffer Cells
|
---|---|
Chapter number | 21 |
Book title |
Hepatitis B Virus
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6700-1_21 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6698-1, 978-1-4939-6700-1
|
Authors |
Jia Liu, Xuan Huang, Melanie Werner, Ruth Broering, Dongliang Yang, Mengji Lu |
Abstract |
Separation of pure cell populations from the liver is a prerequisite to study the role of hepatic parenchymal and non-parenchymal cells in liver physiology, pathophysiology, and immunology. Traditional methods for hepatic cell separation usually purify only single cell types from liver specimens. Here, we describe an efficient method that can simultaneously purify populations of hepatocytes (HCs), liver sinusoidal endothelial cells (LSECs), and Kupffer cells (KCs) from a single mouse liver specimen. A liberase-based perfusion technique in combination with a low-speed centrifugation and magnetic-activated cell sorting (MACS) led to the isolation and purification of HCs, KCs, and LSECs with high yields and purity. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 32 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 7 | 22% |
Student > Master | 5 | 16% |
Researcher | 3 | 9% |
Student > Bachelor | 2 | 6% |
Unspecified | 1 | 3% |
Other | 2 | 6% |
Unknown | 12 | 38% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 6 | 19% |
Immunology and Microbiology | 4 | 13% |
Pharmacology, Toxicology and Pharmaceutical Science | 3 | 9% |
Medicine and Dentistry | 2 | 6% |
Chemical Engineering | 1 | 3% |
Other | 2 | 6% |
Unknown | 14 | 44% |