Chapter title |
An ELISPOT-Based Assay to Measure HBV-Specific CD8 + T Cell Responses in Immunocompetent Mice
|
---|---|
Chapter number | 20 |
Book title |
Hepatitis B Virus
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6700-1_20 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6698-1, 978-1-4939-6700-1
|
Authors |
Tracy D. Reynolds, Safiehkhatoon Moshkani, Michael D. Robek |
Abstract |
Despite some important limitations, immunocompetent mouse models of HBV replication remain an essential tool for studying cellular and humoral immunity to the virus. CD8(+) T cells are a critical component of the immune response to HBV due to their ability to both kill virus-infected hepatocytes and produce cytokines such as IFN-γ that non-cytopathically inhibit virus replication. A number of techniques can be used to measure the magnitude, specificity, and functionality of HBV-specific CD8(+) T cells, each having its own unique advantages. We describe here the enzyme-linked immunospot (ELISPOT)-based assay, which, compared to other methods, is sensitive, cost-effective, and rapid and requires relatively little optimization, specialized training, or equipment. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 6 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Other | 2 | 33% |
Professor > Associate Professor | 1 | 17% |
Researcher | 1 | 17% |
Student > Master | 1 | 17% |
Unknown | 1 | 17% |
Readers by discipline | Count | As % |
---|---|---|
Immunology and Microbiology | 2 | 33% |
Economics, Econometrics and Finance | 1 | 17% |
Agricultural and Biological Sciences | 1 | 17% |
Unknown | 2 | 33% |