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Hepatitis B Virus

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Cover of 'Hepatitis B Virus'

Table of Contents

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    Book Overview
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    Chapter 1 NTCP-Reconstituted In Vitro HBV Infection System
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    Chapter 2 Hepatitis B Virus Infection of HepaRG Cells, HepaRG-hNTCP Cells, and Primary Human Hepatocytes
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    Chapter 3 Live Cell Imaging Confocal Microscopy Analysis of HBV Myr-PreS1 Peptide Binding and Uptake in NTCP-GFP Expressing HepG2 Cells
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    Chapter 4 Intracytoplasmic Transport of Hepatitis B Virus Capsids
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    Chapter 5 A Homokaryon Assay for Nucleocytoplasmic Shuttling Activity of HBV Core Protein
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    Chapter 6 Analyses of HBV cccDNA Quantification and Modification
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    Chapter 7 Detection of HBV cccDNA Methylation from Clinical Samples by Bisulfite Sequencing and Methylation-Specific PCR
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    Chapter 8 A T7 Endonuclease I Assay to Detect Talen-Mediated Targeted Mutation of HBV cccDNA
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    Chapter 9 Detection of Hepatocyte Clones Containing Integrated Hepatitis B Virus DNA Using Inverse Nested PCR
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    Chapter 10 Highly Sensitive Detection of HBV RNA in Liver Tissue by In Situ Hybridization
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    Chapter 11 Immunofluorescent Staining for the Detection of the Hepatitis B Core Antigen in Frozen Liver Sections of Human Liver Chimeric Mice
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    Chapter 12 Measuring Changes in Cytosolic Calcium Levels in HBV- and HBx-Expressing Cultured Primary Hepatocytes
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    Chapter 13 In Vitro Assays for RNA Binding and Protein Priming of Hepatitis B Virus Polymerase
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    Chapter 14 In Vitro Enzymatic and Cell Culture-Based Assays for Measuring Activity of HBV RNaseH Inhibitors
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    Chapter 15 Detection of Hepatitis B Virus Particles Released from Cultured Cells by Particle Gel Assay
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    Chapter 16 Microtiter-Format Assays for HBV Antigen Quantitation in Nonclinical Applications
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    Chapter 17 Deep Sequencing of the Hepatitis B Virus Genome: Analysis of Multiple Samples by Implementation of the Illumina Platform
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    Chapter 18 Generation of Replication-Competent Hepatitis B Virus Genome from Blood Samples for Functional Characterization
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    Chapter 19 Hydrodynamic HBV Transfection Mouse Model
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    Chapter 20 An ELISPOT-Based Assay to Measure HBV-Specific CD8 + T Cell Responses in Immunocompetent Mice
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    Chapter 21 Advanced Method for Isolation of Mouse Hepatocytes, Liver Sinusoidal Endothelial Cells, and Kupffer Cells
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    Chapter 22 Partial Hepatectomy and Castration of HBV Transgenic Mice
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    Chapter 23 Studying HBV Infection and Therapy in Immune-Deficient NOD-Rag1−/−IL2RgammaC-null (NRG) Fumarylacetoacetate Hydrolase (Fah) Knockout Mice Transplanted with Human Hepatocytes
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    Chapter 24 Measurement of Antiviral Effect and Innate Immune Response During Treatment of Primary Woodchuck Hepatocytes
Attention for Chapter 17: Deep Sequencing of the Hepatitis B Virus Genome: Analysis of Multiple Samples by Implementation of the Illumina Platform
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Chapter title
Deep Sequencing of the Hepatitis B Virus Genome: Analysis of Multiple Samples by Implementation of the Illumina Platform
Chapter number 17
Book title
Hepatitis B Virus
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-6700-1_17
Pubmed ID
Book ISBNs
978-1-4939-6698-1, 978-1-4939-6700-1
Authors

Quan-Xin Long, Jie-Li Hu, Ai-Long Huang

Abstract

The quasispecies variation of hepatitis B virus (HBV) was believed to be a viral response to antiviral treatment and host immune pressure. Sanger sequencing was previously the classic approach for quasispecies analysis, but this method was also time-consuming and laborious. Ultra-deep sequencing has been widely used in viral quasispecies research, especially for low-frequency mutation detection. Here we present a multiple samples deep sequencing method employing the Illumina platform to detect HBV quasispecies variation in patient-derived samples.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 11 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 11 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 2 18%
Student > Postgraduate 2 18%
Student > Master 2 18%
Unspecified 1 9%
Lecturer 1 9%
Other 2 18%
Unknown 1 9%
Readers by discipline Count As %
Agricultural and Biological Sciences 3 27%
Medicine and Dentistry 3 27%
Biochemistry, Genetics and Molecular Biology 1 9%
Unspecified 1 9%
Immunology and Microbiology 1 9%
Other 1 9%
Unknown 1 9%