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Nuclear Bodies and Noncoding RNAs

Overview of attention for book
Cover of 'Nuclear Bodies and Noncoding RNAs'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 Visualization of lncRNA by Single-Molecule Fluorescence In Situ Hybridization
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    Chapter 2 Super-Resolution Imaging of Nuclear Bodies by STED Microscopy.
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    Chapter 3 High-Resolution 3D DNA FISH Using Plasmid Probes and Computational Correction of Optical Aberrations to Study Chromatin Structure at the Sub-megabase Scale.
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    Chapter 4 Time-Lapse Imaging of Nuclear Bodies
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    Chapter 5 Visualization of Nucleic Acids with Synthetic Exciton-Controlled Fluorescent Oligonucleotide Probes
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    Chapter 6 Live CLEM Imaging to Analyze Nuclear Structures at High Resolution
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    Chapter 7 Ultrastructural Analysis of Nuclear Bodies Using Electron Microscopy
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    Chapter 8 Analyses of Nuclear Proteins and Nucleic Acid Structures Using Atomic Force Microscopy.
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    Chapter 9 Genome-Wide Co-Localization Screening of Nuclear Body Components Using a Fluorescently Tagged FLJ cDNA Clone Library.
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    Chapter 10 Purification of Specific Chromatin Regions Using Oligonucleotides: Capture Hybridization Analysis of RNA Targets (CHART).
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    Chapter 11 RNA Antisense Purification (RAP) for Mapping RNA Interactions with Chromatin.
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    Chapter 12 In Situ Dissection of RNA Functional Subunits by Domain-Specific Chromatin Isolation by RNA Purification (dChIRP).
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    Chapter 13 Extracting, Enriching, and Identifying Nuclear Body Sub-Complexes Using Label-Based Quantitative Mass Spectrometry.
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    Chapter 14 Studying RNA-Binding Protein Interactions with Target mRNAs in Eukaryotic Cells: Native Ribonucleoprotein Immunoprecipitation (RIP) Assays
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    Chapter 15 Cross-Linking and Immunoprecipitation of Nuclear RNA-Binding Proteins
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    Chapter 16 Purification of Noncoding RNA and Bound Proteins Using FLAG Peptide-Conjugated Antisense-Oligonucleotides.
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    Chapter 17 MMCT-Mediated Chromosome Engineering Technique Applicable to Functional Analysis of lncRNA and Nuclear Dynamics
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    Chapter 18 Reconstitution of Nucleocytoplasmic Transport Using Digitonin-Permeabilized Cells
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    Chapter 19 Genome-Wide Analysis of Long Noncoding RNA Turnover.
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    Chapter 20 Knockdown of Nuclear-Retained Long Noncoding RNAs Using Modified DNA Antisense Oligonucleotides.
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    Chapter 21 siRNA Screening of Nuclear Proteins
Attention for Chapter 8: Analyses of Nuclear Proteins and Nucleic Acid Structures Using Atomic Force Microscopy.
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Chapter title
Analyses of Nuclear Proteins and Nucleic Acid Structures Using Atomic Force Microscopy.
Chapter number 8
Book title
Nuclear Bodies and Noncoding RNAs
Published in
Methods in molecular biology, January 2015
DOI 10.1007/978-1-4939-2253-6_8
Pubmed ID
Book ISBNs
978-1-4939-2252-9, 978-1-4939-2253-6
Authors

Jamie L Gilmore, Aiko Yoshida, Hirohide Takahashi, Katashi Deguchi, Toshiro Kobori, Emilie Louvet, Masahiro Kumeta, Shige H Yoshimura, Kunio Takeyasu, Jamie L. Gilmore, Shige H. Yoshimura

Abstract

Since the inception of atomic force microscopy (AFM) in 1986, the value of this technology for exploring the structure and biophysical properties of a variety of biological samples has been increasingly recognized. AFM provides the opportunity to both image samples at nanometer resolution and also measure the forces on the surface of the sample. Here, we describe a variety of methods for studying nuclear samples including single nucleic acid molecules, higher-order chromatin structures, the nucleolus, and the nucleus. Protocols to prepare nucleic acids, nucleic acid-protein complexes, reconstituted chromatin, the cell nucleus, and the nucleolus are included, as well as protocols describing how to prepare the AFM substrate and the AFM tip. Finally, we describe how to perform conventional imaging, high-speed imaging, recognition imaging, force spectroscopy, and nanoindentation experiments.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 16 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Spain 1 6%
Unknown 15 94%

Demographic breakdown

Readers by professional status Count As %
Researcher 3 19%
Student > Bachelor 3 19%
Student > Ph. D. Student 3 19%
Professor 3 19%
Student > Master 2 13%
Other 2 13%
Readers by discipline Count As %
Agricultural and Biological Sciences 7 44%
Biochemistry, Genetics and Molecular Biology 6 38%
Immunology and Microbiology 1 6%
Social Sciences 1 6%
Medicine and Dentistry 1 6%
Other 0 0%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 04 January 2015.
All research outputs
#3,799,204
of 4,691,823 outputs
Outputs from Methods in molecular biology
#2,172
of 3,382 outputs
Outputs of similar age
#122,549
of 155,996 outputs
Outputs of similar age from Methods in molecular biology
#267
of 401 outputs
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So far Altmetric has tracked 3,382 research outputs from this source. They receive a mean Attention Score of 1.4. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 401 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.