Chapter title |
Analyses of Nuclear Proteins and Nucleic Acid Structures Using Atomic Force Microscopy.
|
---|---|
Chapter number | 8 |
Book title |
Nuclear Bodies and Noncoding RNAs
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2253-6_8 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2252-9, 978-1-4939-2253-6
|
Authors |
Jamie L Gilmore, Aiko Yoshida, Hirohide Takahashi, Katashi Deguchi, Toshiro Kobori, Emilie Louvet, Masahiro Kumeta, Shige H Yoshimura, Kunio Takeyasu, Jamie L. Gilmore, Shige H. Yoshimura |
Abstract |
Since the inception of atomic force microscopy (AFM) in 1986, the value of this technology for exploring the structure and biophysical properties of a variety of biological samples has been increasingly recognized. AFM provides the opportunity to both image samples at nanometer resolution and also measure the forces on the surface of the sample. Here, we describe a variety of methods for studying nuclear samples including single nucleic acid molecules, higher-order chromatin structures, the nucleolus, and the nucleus. Protocols to prepare nucleic acids, nucleic acid-protein complexes, reconstituted chromatin, the cell nucleus, and the nucleolus are included, as well as protocols describing how to prepare the AFM substrate and the AFM tip. Finally, we describe how to perform conventional imaging, high-speed imaging, recognition imaging, force spectroscopy, and nanoindentation experiments. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
France | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Spain | 1 | 5% |
Unknown | 19 | 95% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 3 | 15% |
Professor | 3 | 15% |
Researcher | 3 | 15% |
Student > Ph. D. Student | 3 | 15% |
Other | 2 | 10% |
Other | 4 | 20% |
Unknown | 2 | 10% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 8 | 40% |
Agricultural and Biological Sciences | 6 | 30% |
Unspecified | 1 | 5% |
Immunology and Microbiology | 1 | 5% |
Economics, Econometrics and Finance | 1 | 5% |
Other | 1 | 5% |
Unknown | 2 | 10% |