↓ Skip to main content
What is this page? Embed badge

Nuclear Bodies and Noncoding RNAs

Overview of attention for book
Cover of 'Nuclear Bodies and Noncoding RNAs'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Visualization of lncRNA by Single-Molecule Fluorescence In Situ Hybridization
  3. Altmetric Badge
    Chapter 2 Super-Resolution Imaging of Nuclear Bodies by STED Microscopy.
  4. Altmetric Badge
    Chapter 3 High-Resolution 3D DNA FISH Using Plasmid Probes and Computational Correction of Optical Aberrations to Study Chromatin Structure at the Sub-megabase Scale.
  5. Altmetric Badge
    Chapter 4 Time-Lapse Imaging of Nuclear Bodies
  6. Altmetric Badge
    Chapter 5 Visualization of Nucleic Acids with Synthetic Exciton-Controlled Fluorescent Oligonucleotide Probes
  7. Altmetric Badge
    Chapter 6 Live CLEM Imaging to Analyze Nuclear Structures at High Resolution
  8. Altmetric Badge
    Chapter 7 Ultrastructural Analysis of Nuclear Bodies Using Electron Microscopy
  9. Altmetric Badge
    Chapter 8 Analyses of Nuclear Proteins and Nucleic Acid Structures Using Atomic Force Microscopy.
  10. Altmetric Badge
    Chapter 9 Genome-Wide Co-Localization Screening of Nuclear Body Components Using a Fluorescently Tagged FLJ cDNA Clone Library.
  11. Altmetric Badge
    Chapter 10 Purification of Specific Chromatin Regions Using Oligonucleotides: Capture Hybridization Analysis of RNA Targets (CHART).
  12. Altmetric Badge
    Chapter 11 RNA Antisense Purification (RAP) for Mapping RNA Interactions with Chromatin.
  13. Altmetric Badge
    Chapter 12 In Situ Dissection of RNA Functional Subunits by Domain-Specific Chromatin Isolation by RNA Purification (dChIRP).
  14. Altmetric Badge
    Chapter 13 Extracting, Enriching, and Identifying Nuclear Body Sub-Complexes Using Label-Based Quantitative Mass Spectrometry.
  15. Altmetric Badge
    Chapter 14 Studying RNA-Binding Protein Interactions with Target mRNAs in Eukaryotic Cells: Native Ribonucleoprotein Immunoprecipitation (RIP) Assays
  16. Altmetric Badge
    Chapter 15 Cross-Linking and Immunoprecipitation of Nuclear RNA-Binding Proteins
  17. Altmetric Badge
    Chapter 16 Purification of Noncoding RNA and Bound Proteins Using FLAG Peptide-Conjugated Antisense-Oligonucleotides.
  18. Altmetric Badge
    Chapter 17 MMCT-Mediated Chromosome Engineering Technique Applicable to Functional Analysis of lncRNA and Nuclear Dynamics
  19. Altmetric Badge
    Chapter 18 Reconstitution of Nucleocytoplasmic Transport Using Digitonin-Permeabilized Cells
  20. Altmetric Badge
    Chapter 19 Genome-Wide Analysis of Long Noncoding RNA Turnover.
  21. Altmetric Badge
    Chapter 20 Knockdown of Nuclear-Retained Long Noncoding RNAs Using Modified DNA Antisense Oligonucleotides.
  22. Altmetric Badge
    Chapter 21 siRNA Screening of Nuclear Proteins
Attention for Chapter 13: Extracting, Enriching, and Identifying Nuclear Body Sub-Complexes Using Label-Based Quantitative Mass Spectrometry.
Altmetric Badge

Mentioned by

twitter
1 X user

Citations

dimensions_citation
4 Dimensions

Readers on

mendeley
2 Mendeley
Summary X Dimensions citations
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Chapter title
Extracting, Enriching, and Identifying Nuclear Body Sub-Complexes Using Label-Based Quantitative Mass Spectrometry.
Chapter number 13
Book title
Nuclear Bodies and Noncoding RNAs
Published in
Methods in molecular biology, January 2015
DOI 10.1007/978-1-4939-2253-6_13
Pubmed ID
25555584
Book ISBNs
978-1-4939-2252-9, 978-1-4939-2253-6
Authors

Fox A, Mehta V, Boulon S, Trinkle-Mulcahy L, Archa Fox, Virja Mehta, Severine Boulon, Laura Trinkle-Mulcahy

Abstract

Determining the proteome of a nuclear body is a crucial step toward understanding its function; however, it is extremely challenging to obtain pure nuclear body preparations. Moreover, many nuclear proteins dynamically associate with multiple bodies and subnuclear compartments, confounding analysis. We have found that a more practical approach is to carry out affinity purification of nuclear body sub-complexes via the use of tagged nuclear-body-specific marker proteins. Here we describe in detail the method to identify new nuclear body protein sub-complexes through SILAC (stable isotope labeling by amino acids in culture)-based affinity purification followed by quantitative mass spectrometry.

View on publisher site
X Demographics

X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 2 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Canada 1 50%
Unknown 1 50%

Demographic breakdown

Readers by professional status Count As %
Student > Master 1 50%
Unknown 1 50%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 1 50%
Unknown 1 50%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 06 January 2015.
All research outputs
#20,248,338
of 22,776,824 outputs
Outputs from Methods in molecular biology
#9,866
of 13,092 outputs
Outputs of similar age
#295,855
of 353,002 outputs
Outputs of similar age from Methods in molecular biology
#641
of 1,009 outputs
Altmetric has tracked 22,776,824 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,092 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 353,002 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 1,009 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.

This page is provided by Altmetric.