Chapter title |
Enzyme-Linked ImmunoSpot (ELISpot) for Single-Cell Analysis
|
---|---|
Chapter number | 3 |
Book title |
Single Cell Protein Analysis
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2987-0_3 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2986-3, 978-1-4939-2987-0
|
Authors |
Sylvia Janetzki, Rachel Rabin |
Abstract |
The ELISpot, a heterogeneous immunoassay, is widely used for detection of low abundant analytes. It is a reliable and robust assay to monitor responses of the immune system at the single-cell level by capturing secreted molecules of interest with specific, membrane-bound antibodies. Those molecules are then made visible by a cascade of ELISA-related development steps. The final results are distinct spots on the membrane as an imprint of the cell secreting the captured molecules, not only allowing their quantification but also providing insight on the kinetics and strength of secretion. This chapter describes the optimized protocol steps of the ELISpot technique, important improvements and tools available for the community, and the current expansion of the technique into polyfunctional cell analysis. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 11 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 3 | 27% |
Student > Doctoral Student | 2 | 18% |
Researcher | 1 | 9% |
Student > Master | 1 | 9% |
Unknown | 4 | 36% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 3 | 27% |
Agricultural and Biological Sciences | 1 | 9% |
Medicine and Dentistry | 1 | 9% |
Chemistry | 1 | 9% |
Engineering | 1 | 9% |
Other | 0 | 0% |
Unknown | 4 | 36% |