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Mendeley readers
Chapter title |
Ultrasensitive Protein Detection on Dot Blots and Western Blots with Semiconducting Polymer Dots
|
---|---|
Chapter number | 14 |
Book title |
Detection of Blotted Proteins
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Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2718-0_14 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2717-3, 978-1-4939-2718-0
|
Authors |
Fangmao Ye, Polina B. Smith, Daniel T. Chiu, Ye, Fangmao, Smith, Polina B., Chiu, Daniel T. |
Abstract |
We demonstrate ultrasensitive fluorescence imaging of proteins on dot blots and western blots using a bright, compact, and orange-emitting semiconducting polymer dot (CNPPV). We achieved a detection limit at the single-picogram level in dot blots; with conventional western blot, we detected 50 pg of transferrin and trypsin inhibitor after SDS-PAGE and transfer onto a PVDF membrane. In this protocol, we describe the preparation of Pdots and its streptavidin bioconjugate and the procedures of dot blot and western blots. |
Mendeley readers
The data shown below were compiled from readership statistics for 12 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 12 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 4 | 33% |
Student > Ph. D. Student | 2 | 17% |
Unspecified | 1 | 8% |
Unknown | 5 | 42% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 2 | 17% |
Materials Science | 2 | 17% |
Unspecified | 1 | 8% |
Agricultural and Biological Sciences | 1 | 8% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 8% |
Other | 0 | 0% |
Unknown | 5 | 42% |