Chapter title |
Extraction and Purification of R-phycoerythrin from Marine Red Algae
|
---|---|
Chapter number | 5 |
Book title |
Natural Products From Marine Algae
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2684-8_5 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2683-1, 978-1-4939-2684-8
|
Authors |
Justine Dumay, Michèle Morançais, Huu Phuo Trang Nguyen, Joël Fleurence, Dumay, Justine, Morançais, Michèle, Nguyen, Huu Phuo Trang, Fleurence, Joël |
Editors |
Dagmar B. Stengel, Solène Connan |
Abstract |
This chapter focuses on the recovery of an R-Phycoerythrin (R-PE)-enriched fraction from marine algae. Since R-PE is a proteinaceous pigment, we have developed a simple and rapid two-step method devoted to the extraction and purification of R-PE from marine red algae. Here we describe a phosphate buffer extraction followed by anion exchange chromatography carried on a DEAE Sepharose Fast Flow column. To ensure the quality and quantity of R-PE recovery, we also indicate different methods to monitor each fraction obtained, such as spectrophotometric indicators, gel filtration, and SDS-PAGE analysis. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Norway | 1 | 2% |
Unknown | 48 | 98% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 9 | 18% |
Student > Master | 7 | 14% |
Researcher | 7 | 14% |
Student > Ph. D. Student | 7 | 14% |
Student > Doctoral Student | 2 | 4% |
Other | 6 | 12% |
Unknown | 11 | 22% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 15 | 31% |
Biochemistry, Genetics and Molecular Biology | 8 | 16% |
Environmental Science | 4 | 8% |
Unspecified | 2 | 4% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 2% |
Other | 4 | 8% |
Unknown | 15 | 31% |