| Chapter title |
Characterization of Alginates by Nuclear Magnetic Resonance (NMR) and Vibrational Spectroscopy (IR, NIR, Raman) in Combination with Chemometrics
|
|---|---|
| Chapter number | 22 |
| Book title |
Natural Products From Marine Algae
|
| Published in |
Methods in molecular biology, January 2015
|
| DOI | 10.1007/978-1-4939-2684-8_22 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-2683-1, 978-1-4939-2684-8
|
| Authors |
Henrik Max Jensen, Flemming Hofmann Larsen, Søren Balling Engelsen |
| Editors |
Dagmar B. Stengel, Solène Connan |
| Abstract |
This chapter describes three different spectroscopic methods for structural characterization of the commercial important hydrocolloid alginate extracted from brown seaweed. The "golden" reference method for characterization of the alginate structure is (1)H liquid-state NMR of depolymerized alginate polymers using a stepwise hydrolysis. Having implemented this method, predictive and rapid non-destructive methods using vibrational spectroscopy and chemometrics can be developed. These methods can predict the M/G-ratio of the intact alginate powder with at least the same precision and accuracy as the reference method in a fraction of the time that is required to measure the alginate using the reference method. The chapter also demonstrates how solid-state (13)C CP/MAS NMR can be used to determine the M/G ratio on the intact sample by the use of multivariate chemometrics and how this method shares the characteristics of the solid-state non-destructive IR method rather than its liquid-state counterpart. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 67 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 10 | 15% |
| Student > Master | 9 | 13% |
| Student > Doctoral Student | 7 | 10% |
| Student > Bachelor | 6 | 9% |
| Professor | 4 | 6% |
| Other | 9 | 13% |
| Unknown | 22 | 33% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 9 | 13% |
| Engineering | 8 | 12% |
| Pharmacology, Toxicology and Pharmaceutical Science | 7 | 10% |
| Biochemistry, Genetics and Molecular Biology | 5 | 7% |
| Agricultural and Biological Sciences | 5 | 7% |
| Other | 12 | 18% |
| Unknown | 21 | 31% |