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Chapter title |
Virus-Induced Gene Silencing for Gene Function Studies in Barley.
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Chapter number | 22 |
Book title |
Environmental Responses in Plants
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Published in |
Methods in molecular biology, January 2016
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DOI | 10.1007/978-1-4939-3356-3_22 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3354-9, 978-1-4939-3356-3
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Authors |
Maria Barciszewska-Pacak, Artur Jarmołowski, Andrzej Pacak |
Editors |
Paula Duque |
Abstract |
Virus-Induced Gene Silencing (VIGS) creates a natural antiviral defense in plants. However, it has been also a powerful tool for endogenous gene silencing in dicot and monocot plants by exploitation of recombinant viruses, harboring silencing inducing sequences. The Barley Stripe Mosaic Virus (BSMV) based VIGS system is an efficient and rapid RNAi approach that is routinely applied in functional genomics studies of cereals. We present here a protocol for BSMV VIGS application in barley based on mechanical inoculation of the plants with in vitro transcribed recombinant BSMV RNAs as the silencing triggers. |
Mendeley readers
The data shown below were compiled from readership statistics for 6 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 6 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Doctoral Student | 3 | 50% |
Researcher | 2 | 33% |
Unknown | 1 | 17% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 5 | 83% |
Unknown | 1 | 17% |