Chapter title |
ELISA
|
---|---|
Chapter number | 10 |
Book title |
ELISA
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2742-5_10 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2741-8, 978-1-4939-2742-5
|
Authors |
Chennuru, Sreedevi, Pavuluri, Panduranga Rao, Sreedevi Chennuru, Panduranga Rao Pavuluri |
Abstract |
Flow-through assay (FTA) is a rapid, simple-to-perform, cost-effective, and user-friendly diagnostic test for monitoring infections in non-laboratory settings. It is mostly applied for antibody detection. FTA employing protein-A colloidal gold conjugate to detect antibodies against porcine cysticerci using cyst fluid and whole cyst antigens of Taenia solium metacestode is described here. Antibodies in the serum are captured by an antigen spotted onto a nitrocellulose membrane mounted on a flow-through device that serves as the antigen capture matrix. The bound antibodies are visualized by the addition of protein-A colloidal gold conjugate, which imparts a pink color. The test can be completed within 3 min at room temperature without any instrumentation. The sensitivity and specificity of the FTA are in agreement with ELISA. |
X Demographics
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United Kingdom | 1 | 100% |
Demographic breakdown
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
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Unknown | 14 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Bachelor | 3 | 21% |
Student > Ph. D. Student | 3 | 21% |
Researcher | 2 | 14% |
Student > Master | 1 | 7% |
Other | 1 | 7% |
Other | 2 | 14% |
Unknown | 2 | 14% |
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Medicine and Dentistry | 2 | 14% |
Agricultural and Biological Sciences | 1 | 7% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 7% |
Other | 2 | 14% |
Unknown | 3 | 21% |