Chapter title |
The Nuclear Envelope
|
---|---|
Chapter number | 6 |
Book title |
The Nuclear Envelope
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3530-7_6 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3528-4, 978-1-4939-3530-7
|
Authors |
Mudumbi, Krishna C, Yang, Weidong, Krishna C. Mudumbi, Weidong Yang |
Editors |
Sue Shackleton, Philippe Collas, Eric C. Schirmer |
Abstract |
Determining the locations of nuclear envelope transmembrane proteins and their concentrations across the outer and inner nuclear membranes has been a challenging and time-consuming process. Typically, this required the week-long process of fixing and immunogold staining of cells prior to analysis by electron microscopy. Here, we describe a method, single-point fluorescence recovery after photobleaching (spFRAP), which is able to quickly determine the localization and distribution of nuclear membrane proteins along the double nuclear envelope membranes with a precision of 10-15 nm in a matter of 10-20 min the day after transfection. |
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