| Chapter title |
In Situ Detection of Interactions Between Nuclear Envelope Proteins and Partners.
|
|---|---|
| Chapter number | 9 |
| Book title |
The Nuclear Envelope
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3530-7_9 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3528-4, 978-1-4939-3530-7
|
| Authors |
Alice Barateau, Brigitte Buendia |
| Editors |
Sue Shackleton, Philippe Collas, Eric C. Schirmer |
| Abstract |
Proximity ligation assay (PLA) appears as a quick and easy technique to visualize within fixed cells the occurrence and in situ distribution of protein complexes. PLA has been validated to detect protein-protein interactions within the nuclear compartment. Here, we describe a protocol which allows the detection of interactions between A-type nuclear lamins and either LEM-domain proteins (such as emerin, integrated within the inner nuclear membrane, and LAP2α which accumulates within the nucleoplasm) or gene regulatory factors (e.g., the transcription factor SREBP1). The distinct amounts and patterns of PLA signals obtained for various complexes highlight the pertinence of using PLA to reveal in situ where and to which extent nuclear envelope proteins bind specific partners. |
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Geographical breakdown
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|---|---|---|
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Demographic breakdown
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|---|---|---|
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| Other | 1 | 20% |
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|---|---|---|
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