Chapter title |
Exploring the Protein Composition of the Plant Nuclear Envelope.
|
---|---|
Chapter number | 2 |
Book title |
The Nuclear Envelope
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3530-7_2 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3528-4, 978-1-4939-3530-7
|
Authors |
Xiao Zhou, Kentaro Tamura, Katja Graumann, Iris Meier |
Editors |
Sue Shackleton, Philippe Collas, Eric C. Schirmer |
Abstract |
Due to rather limited sequence similarity, targeted identification of plant nuclear envelope and nuclear pore complex proteins has mainly followed two routes: (1) advanced computational identification followed by experimental verification and (2) immunoaffinity purification of complexes followed by mass spectrometry. Following candidate identification, fluorescence recovery after photobleaching (FRAP) and fluorescence resonance energy transfer (FRET) provide powerful tools to verify protein-protein interactions in situ at the NE. Here, we describe these methods for the example of Arabidopsis thaliana nuclear pore and nuclear envelope protein identification. |
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