| Chapter title |
Plant Proteostasis
|
|---|---|
| Chapter number | 22 |
| Book title |
Plant Proteostasis
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3759-2_22 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3757-8, 978-1-4939-3759-2
|
| Authors |
Carretero-Paulet, Lorenzo, Albert, Victor A, Lorenzo Carretero-Paulet, Victor A. Albert |
| Editors |
L. Maria Lois, Rune Matthiesen |
| Abstract |
Molecular evolutionary analysis of gene families commonly involves a sequence of steps including multiple sequence alignment (MSA) and reconstructing phylogenetic trees, using any of the multiple algorithms available. SeaView is a multiplatform program that integrates different methods for performing the above tasks, and others, within a friendly and simple-to-use graphical user interface (Gouy et al. Mol Biol Evol 27(2):221-224, 2010). By using SeaView, we will investigate the evolutionary relationships among SAE1 genes in Brassicaceae species by means of two alternative methods of phylogenetic reconstruction: Maximum Likelihood (ML) and Neighbor-Joining (NJ). Prior to ML phylogenetic analysis (Guindon and Gascuel. Syst Biol 52(5):696-704, 2003), we will use ProtTest to select the best-fit evolutionary model of amino acid substitution for the MSA of SAE1 proteins (Abascal et al. Bioinformatics 21(9):2104-2105, 2005). |
X Demographics
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| Unknown | 2 | 100% |
Demographic breakdown
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| Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 5 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
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| Professor > Associate Professor | 1 | 20% |
| Other | 1 | 20% |
| Unknown | 3 | 60% |
| Readers by discipline | Count | As % |
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| Biochemistry, Genetics and Molecular Biology | 2 | 40% |
| Agricultural and Biological Sciences | 1 | 20% |
| Unknown | 2 | 40% |