| Chapter title |
The Mitotic Spindle
|
|---|---|
| Chapter number | 6 |
| Book title |
The Mitotic Spindle
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3542-0_6 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3540-6, 978-1-4939-3542-0
|
| Authors |
King, Matthew, Petry, Sabine, Matthew King, Sabine Petry |
| Editors |
Paul Chang, Ryoma Ohi |
| Abstract |
Mitotic and meiotic spindles consist primarily of microtubules, which originate from centrosomes and within the vicinity of chromatin. Indirect evidence suggested that microtubules also originate throughout the spindle, but the high microtubule density within the spindle precludes the direct observation of this phenomenon. By using meiotic Xenopus laevis egg extract and employing total internal reflection (TIRF) microscopy, microtubule nucleation from preexisting microtubules could be demonstrated and analyzed. Branching microtubule nucleation is an ideal mechanism to assemble and maintain a mitotic spindle, because microtubule numbers are amplified while preserving their polarity. Here, we describe the assays that made these findings possible and the experiments that helped identify the key molecular players involved. |
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Demographic breakdown
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Mendeley readers
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