| Chapter title |
Measuring the Effects of Microtubule-Associated Proteins on Microtubule Dynamics In Vitro.
|
|---|---|
| Chapter number | 4 |
| Book title |
The Mitotic Spindle
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3542-0_4 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3540-6, 978-1-4939-3542-0
|
| Authors |
Marija Zanic |
| Editors |
Paul Chang, Ryoma Ohi |
| Abstract |
Microtubule dynamic instability, the process by which individual microtubules switch between phases of growth and shrinkage, is essential for establishing the architecture of cellular microtubule structures, such as the mitotic spindle. This switching process is regulated by a complex network of microtubule-associated proteins (MAPs), which modulate different aspects of microtubule dynamic behavior. To elucidate the effects of MAPs and their molecular mechanisms of action, in vitro reconstitution approaches with purified components are used. Here, I present methods for measuring individual and combined effects of MAPs on microtubule dynamics, using purified protein components and total-internal-reflection fluorescence (TIRF) microscopy. Particular focus is given to the experimental design, proper parameterization, and data analysis. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 30 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 6 | 20% |
| Student > Doctoral Student | 4 | 13% |
| Student > Bachelor | 4 | 13% |
| Student > Ph. D. Student | 4 | 13% |
| Student > Master | 3 | 10% |
| Other | 3 | 10% |
| Unknown | 6 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 10 | 33% |
| Agricultural and Biological Sciences | 7 | 23% |
| Neuroscience | 3 | 10% |
| Physics and Astronomy | 1 | 3% |
| Computer Science | 1 | 3% |
| Other | 0 | 0% |
| Unknown | 8 | 27% |