Report for: The Mitotic Spindle

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Chapter title	The Mitotic Spindle
Chapter number	12
Book title	The Mitotic Spindle
Published in	Methods in molecular biology, January 2016
DOI	10.1007/978-1-4939-3542-0_12
Pubmed ID	27193850
Book ISBNs	978-1-4939-3540-6, 978-1-4939-3542-0
Authors	Fong, Kimberly K, Graczyk, Beth, Davis, Trisha N, Kimberly K. Fong, Beth Graczyk, Trisha N. Davis
Editors	Paul Chang, Ryoma Ohi
Abstract	Centrosomes are components of the mitotic spindle responsible for organizing microtubules and establishing a bipolar spindle for accurate chromosome segregation. In budding yeast, Saccharomyces cerevisiae, the centrosome is called the spindle pole body, a highly organized trilaminar structure embedded in the nuclear envelope. Here we describe a detailed protocol for the purification of fluorescently labeled spindle pole bodes from S. cerevisiae. Spindle pole bodies are purified from yeast using a TAP-tag purification followed by velocity sedimentation.This highly reproducible TAP-tag purification method improves upon previous techniques and expands the scope of in vitro characterization of yeast spindle pole bodies. The genetic flexibility of this technique allows for the study of spindle pole body mutants as well as the study of spindle pole bodies during different stages of the cell cycle. The ease and reproducibility of the technique make it possible to study spindle pole bodies using a variety of biochemical, biophysical, and microscopic techniques.

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Geographical breakdown

Country	Count	As %
France	1	100%

Demographic breakdown

Type	Count	As %
Members of the public	1	100%

Mendeley readers

The data shown below were compiled from readership statistics for 6 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country	Count	As %
Unknown	6	100%

Demographic breakdown

Readers by professional status	Count	As %
Student > Ph. D. Student	3	50%
Researcher	2	33%
Student > Doctoral Student	1	17%

Readers by discipline	Count	As %
Agricultural and Biological Sciences	4	67%
Biochemistry, Genetics and Molecular Biology	2	33%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 May 2016.

All research outputs

#20,328,845

of 22,873,031 outputs

Outputs from Methods in molecular biology

#9,915

of 13,130 outputs

Outputs of similar age

#330,715

of 393,675 outputs

Outputs of similar age from Methods in molecular biology

#1,054

of 1,471 outputs

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So far Altmetric has tracked 13,130 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.

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The Mitotic Spindle

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Demographic breakdown

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Geographical breakdown

Demographic breakdown

Attention Score in Context