| Chapter title |
Studying Kinetochores In Vivo Using FLIM-FRET.
|
|---|---|
| Chapter number | 11 |
| Book title |
The Mitotic Spindle
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3542-0_11 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3540-6, 978-1-4939-3542-0
|
| Authors |
Tae Yeon Yoo, Daniel J. Needleman |
| Editors |
Paul Chang, Ryoma Ohi |
| Abstract |
Kinetochores play essential roles in coordinating mitosis, as a mechanical connector between chromosome and microtubule and as a source of numerous biochemical signals. These mechanical and biochemical behaviors of kinetochores change dynamically in cells during mitosis. Therefore, understanding kinetochore function requires an imaging tool that quantifies the protein-protein interactions or biochemical changes with high spatiotemporal resolution. FRET has previously been used in combination with biosensors to probe protein-protein interactions and biochemical activity. In this chapter, we introduce FLIM-FRET, a lifetime-based method that quantifies FRET, and describe the use of FLIM-FRET as a method for studying dynamic kinetochore behavior in cells with high spatiotemporal resolution. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 9 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 4 | 44% |
| Student > Bachelor | 1 | 11% |
| Student > Postgraduate | 1 | 11% |
| Unknown | 3 | 33% |
| Readers by discipline | Count | As % |
|---|---|---|
| Physics and Astronomy | 3 | 33% |
| Engineering | 2 | 22% |
| Agricultural and Biological Sciences | 1 | 11% |
| Unknown | 3 | 33% |