Chapter title |
Immobilization Techniques of Bacteria for Live Super-resolution Imaging Using Structured Illumination Microscopy.
|
---|---|
Chapter number | 12 |
Book title |
Bacterial Pathogenesis
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6673-8_12 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6671-4, 978-1-4939-6673-8
|
Authors |
Amy L. Bottomley, Lynne Turnbull, Cynthia B. Whitchurch, Elizabeth J. Harry, Bottomley, Amy L., Turnbull, Lynne, Whitchurch, Cynthia B., Harry, Elizabeth J. |
Editors |
Pontus Nordenfelt, Mattias Collin |
Abstract |
Advancements in optical microscopy technology have allowed huge progression in the ability to understand protein structure and dynamics in live bacterial cells using fluorescence microscopy. Paramount to high-quality microscopy is good sample preparation to avoid bacterial cell movement that can result in motion blur during image acquisition. Here, we describe two techniques of sample preparation that reduce unwanted cell movement and are suitable for application to a number of bacterial species and imaging methods. |
Mendeley readers
Geographical breakdown
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Unknown | 19 | 90% |
Demographic breakdown
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Student > Ph. D. Student | 6 | 29% |
Researcher | 4 | 19% |
Student > Bachelor | 3 | 14% |
Student > Doctoral Student | 1 | 5% |
Student > Master | 1 | 5% |
Other | 3 | 14% |
Unknown | 3 | 14% |
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Chemical Engineering | 1 | 5% |
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