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Bacterial Pathogenesis

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Cover of 'Bacterial Pathogenesis'

Table of Contents

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    Book Overview
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    Chapter 1 Protein-Based Strategies to Identify and Isolate Bacterial Virulence Factors.
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    Chapter 2 Analysis of Bacterial Surface Interactions with Mass Spectrometry-Based Proteomics.
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    Chapter 3 Differential Radial Capillary Action of Ligand Assay (DRaCALA) for High-Throughput Detection of Protein-Metabolite Interactions in Bacteria.
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    Chapter 4 Identifying Bacterial Immune Evasion Proteins Using Phage Display.
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    Chapter 5 Competition for Iron Between Host and Pathogen: A Structural Case Study on Helicobacter pylori.
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    Chapter 6 Common Challenges in Studying the Structure and Function of Bacterial Proteins: Case Studies from Helicobacter pylori.
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    Chapter 7 Development of a Single Locus Sequence Typing (SLST) Scheme for Typing Bacterial Species Directly from Complex Communities.
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    Chapter 8 Reconstructing the Ancestral Relationships Between Bacterial Pathogen Genomes.
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    Chapter 9 Making Fluorescent Streptococci and Enterococci for Live Imaging.
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    Chapter 10 Computer Vision-Based Image Analysis of Bacteria.
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    Chapter 11 Assessing Vacuolar Escape of Listeria Monocytogenes.
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    Chapter 12 Immobilization Techniques of Bacteria for Live Super-resolution Imaging Using Structured Illumination Microscopy.
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    Chapter 13 Negative Staining and Transmission Electron Microscopy of Bacterial Surface Structures.
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    Chapter 14 Detection of Intracellular Proteins by High-Resolution Immunofluorescence Microscopy in Streptococcus pyogenes.
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    Chapter 15 Antibody Guided Molecular Imaging of Infective Endocarditis.
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    Chapter 16 The Zebrafish as a Model for Human Bacterial Infections.
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    Chapter 17 Determining Platelet Activation and Aggregation in Response to Bacteria.
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    Chapter 18 Killing Bacteria with Cytotoxic Effector Proteins of Human Killer Immune Cells: Granzymes, Granulysin, and Perforin.
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    Chapter 19 In Vitro and In Vivo Biofilm Formation by Pathogenic Streptococci.
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    Chapter 20 Murine Mycobacterium marinum Infection as a Model for Tuberculosis.
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    Chapter 21 Generating and Purifying Fab Fragments from Human and Mouse IgG Using the Bacterial Enzymes IdeS, SpeB and Kgp.
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    Chapter 22 Measuring Antibody Orientation at the Bacterial Surface.
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    Chapter 23 Toward Clinical use of the IgG Specific Enzymes IdeS and EndoS against Antibody-Mediated Diseases.
Attention for Chapter 10: Computer Vision-Based Image Analysis of Bacteria.
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Chapter title
Computer Vision-Based Image Analysis of Bacteria.
Chapter number 10
Book title
Bacterial Pathogenesis
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-6673-8_10
Pubmed ID
Book ISBNs
978-1-4939-6671-4, 978-1-4939-6673-8
Authors

Jonas Danielsen, Pontus Nordenfelt

Editors

Pontus Nordenfelt, Mattias Collin

Abstract

Microscopy is an essential tool for studying bacteria, but is today mostly used in a qualitative or possibly semi-quantitative manner often involving time-consuming manual analysis. It also makes it difficult to assess the importance of individual bacterial phenotypes, especially when there are only subtle differences in features such as shape, size, or signal intensity, which is typically very difficult for the human eye to discern. With computer vision-based image analysis - where computer algorithms interpret image data - it is possible to achieve an objective and reproducible quantification of images in an automated fashion. Besides being a much more efficient and consistent way to analyze images, this can also reveal important information that was previously hard to extract with traditional methods. Here, we present basic concepts of automated image processing, segmentation and analysis that can be relatively easy implemented for use with bacterial research.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 21 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Portugal 1 5%
Denmark 1 5%
Unknown 19 90%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 5 24%
Student > Master 4 19%
Professor > Associate Professor 3 14%
Librarian 1 5%
Professor 1 5%
Other 3 14%
Unknown 4 19%
Readers by discipline Count As %
Computer Science 3 14%
Engineering 3 14%
Agricultural and Biological Sciences 2 10%
Immunology and Microbiology 2 10%
Medicine and Dentistry 2 10%
Other 4 19%
Unknown 5 24%