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Drosophila

Overview of attention for book
Cover of 'Drosophila'

Table of Contents

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    Book Overview
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    Chapter 1 Using FlyBase, a Database of Drosophila Genes and Genomes.
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    Chapter 2 The GAL4 System: A Versatile System for the Manipulation and Analysis of Gene Expression.
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    Chapter 3 The Q-System: A Versatile Expression System for Drosophila.
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    Chapter 4 Analysis of MicroRNA Function in Drosophila.
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    Chapter 5 Methods for High-Throughput RNAi Screening in Drosophila Cells.
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    Chapter 6 A Guide to Genome-Wide In Vivo RNAi Applications in Drosophila.
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    Chapter 7 Creating Heritable Mutations in Drosophila with CRISPR-Cas9.
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    Chapter 8 Performing Chromophore-Assisted Laser Inactivation in Drosophila Embryos Using GFP.
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    Chapter 9 deGradFP: A System to Knockdown GFP-Tagged Proteins.
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    Chapter 10 Drosophila
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    Chapter 11 Cultivation and Live Imaging of Drosophila Imaginal Discs.
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    Chapter 12 Cultivation and Live Imaging of Drosophila Ovaries.
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    Chapter 13 Segmentation and Quantitative Analysis of Epithelial Tissues.
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    Chapter 14 Laser Ablation to Probe the Epithelial Mechanics in Drosophila.
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    Chapter 15 Rapid Ovary Mass-Isolation (ROMi) to Obtain Large Quantities of Drosophila Egg Chambers for Fluorescent In Situ Hybridization.
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    Chapter 16 Drosophila
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    Chapter 17 Protocols to Study Growth and Metabolism in Drosophila.
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    Chapter 18 Protocols to Study Aging in Drosophila.
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    Chapter 19 Protocols to Study Behavior in Drosophila.
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    Chapter 20 Electrophysiological Recordings from Lobula Plate Tangential Cells in Drosophila.
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    Chapter 21 Methods to Establish Drosophila Cell Lines.
  23. Altmetric Badge
    Chapter 22 Erratum to: The GAL4 System: A Versatile System for the Manipulation and Analysis of Gene Expression
Attention for Chapter 7: Creating Heritable Mutations in Drosophila with CRISPR-Cas9.
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Chapter title
Creating Heritable Mutations in Drosophila with CRISPR-Cas9.
Chapter number 7
Book title
Drosophila
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-6371-3_7
Pubmed ID
Book ISBNs
978-1-4939-6369-0, 978-1-4939-6371-3
Authors

Fillip Port, Simon L. Bullock, Port, Fillip, Bullock, Simon L.

Editors

Christian Dahmann

Abstract

Reverse genetics-the creation of mutations in preselected target genes-has until recently been a bottleneck in many Drosophila projects. The advent of clustered, regularly interspaced, short palindromic repeat (CRISPR) genome engineering systems has transformed this situation. A short time after the in vitro demonstration of target site cleavage by the RNA-guided endonuclease CRISPR-associated nuclease 9 (Cas9) (Jinek et al., Science 337:816-821, 2012), hundreds of fly researchers are using CRISPR technology to generate loss-of-function mutant alleles in specific genes, as well as to create specific point mutations or tagged protein products. It appears that most target genes can be edited with remarkably high efficiency, with engineered strains often available a few weeks after conception of a project. Here, we provide a step-by-step protocol for creating loss-of-function mutations in Drosophila using transgenic Cas9 sources, which is based on optimized reagents and procedures that have been evaluated in our laboratory. We also provide guidance on extending this protocol to produce precise genomic alterations by homology-directed repair in the presence of a donor sequence. Additional information and updates are available from our website, www.crisprflydesign.org .

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 40 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Netherlands 1 3%
Germany 1 3%
Unknown 38 95%

Demographic breakdown

Readers by professional status Count As %
Researcher 14 35%
Student > Ph. D. Student 7 18%
Student > Bachelor 5 13%
Professor 4 10%
Student > Master 2 5%
Other 3 8%
Unknown 5 13%
Readers by discipline Count As %
Agricultural and Biological Sciences 15 38%
Biochemistry, Genetics and Molecular Biology 12 30%
Neuroscience 2 5%
Medicine and Dentistry 2 5%
Unspecified 1 3%
Other 3 8%
Unknown 5 13%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 January 2018.
All research outputs
#11,945,619
of 15,005,480 outputs
Outputs from Methods in molecular biology
#4,536
of 8,845 outputs
Outputs of similar age
#191,905
of 269,854 outputs
Outputs of similar age from Methods in molecular biology
#5
of 8 outputs
Altmetric has tracked 15,005,480 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 8,845 research outputs from this source. They receive a mean Attention Score of 2.6. This one is in the 29th percentile – i.e., 29% of its peers scored the same or lower than it.
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We're also able to compare this research output to 8 others from the same source and published within six weeks on either side of this one. This one has scored higher than 3 of them.