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Mendeley readers
Chapter title |
In Vitro Assembly of Nucleosomes for Binding/Remodeling Assays.
|
---|---|
Chapter number | 1 |
Book title |
Histones
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Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6630-1_1 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6628-8, 978-1-4939-6630-1
|
Authors |
Arnob Dutta, Jerry L. Workman |
Editors |
Benoit Guillemette, Luc R. Gaudreau |
Abstract |
In order to study the functions and activities of chromatin remodeling enzymes in vitro, it is necessary to be able to reconstitute nucleosomes on DNA templates. In this chapter, we describe procedures for purification of histones from E. coli, formation of octamers, and reconstitution of nucleosomes that can be further modified by chromatin modifiers. In addition, we describe methods to purify nucleosomes from human cells. Finally, we also describe assays to test binding and remodeling activities of chromatin remodelers. |
Mendeley readers
The data shown below were compiled from readership statistics for 8 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 8 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 2 | 25% |
Researcher | 2 | 25% |
Student > Doctoral Student | 2 | 25% |
Student > Postgraduate | 1 | 13% |
Unknown | 1 | 13% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 5 | 63% |
Agricultural and Biological Sciences | 2 | 25% |
Unknown | 1 | 13% |