| Chapter title |
Acid-Urea Gel Electrophoresis and Western Blotting of Histones.
|
|---|---|
| Chapter number | 11 |
| Book title |
Histones
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6630-1_11 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6628-8, 978-1-4939-6630-1
|
| Authors |
Catherine A. Hazzalin, Louis C. Mahadevan |
| Editors |
Benoit Guillemette, Luc R. Gaudreau |
| Abstract |
Acid-urea gel electrophoresis offers significant advantages over SDS-PAGE for analysis of post-translational protein modifications, being capable of resolving proteins of similar size but varying in charge. Hence, it can be used to separate protein variants with small charge-altering differences in primary sequence, and is particularly useful in the analysis of histones whose charge variation arises from post-translational modification, such as phosphorylation or acetylation. On acid-urea gels, histones that carry multiple modifications, each with a characteristic charge, are resolved into distinct bands, the so-called "histone ladder." Thus, the extent and distribution of different modification states of histones can be visualized. Here, we describe the analysis of histone H3 by acid-urea gel electrophoresis and western blotting. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 20 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 4 | 20% |
| Student > Bachelor | 3 | 15% |
| Student > Master | 2 | 10% |
| Student > Doctoral Student | 1 | 5% |
| Unspecified | 1 | 5% |
| Other | 2 | 10% |
| Unknown | 7 | 35% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 7 | 35% |
| Agricultural and Biological Sciences | 2 | 10% |
| Environmental Science | 1 | 5% |
| Unspecified | 1 | 5% |
| Mathematics | 1 | 5% |
| Other | 1 | 5% |
| Unknown | 7 | 35% |