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Histones

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Cover of 'Histones'

Table of Contents

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    Book Overview
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    Chapter 1 In Vitro Assembly of Nucleosomes for Binding/Remodeling Assays.
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    Chapter 2 An Assay for Measuring Histone Variant Exchange within Nucleosomes In Vitro.
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    Chapter 3 Purification of Yeast Native Reagents for the Analysis of Chromatin Function-I: Nucleosomes for Reconstitution and Manipulation of Histone Marks.
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    Chapter 4 Purification of Yeast Native Reagents for the Analysis of Chromatin Function-II: Multiprotein Complexes and Biochemical Assays.
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    Chapter 5 Histone Purification from Saccharomyces cerevisiae.
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    Chapter 6 Analytical Ultracentrifuge Analysis of Nucleosomes Assembled from Recombinant, Acid-Extracted, HPLC-Purified Histones.
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    Chapter 7 SILAC-Based Quantitative Strategies for Accurate Histone Posttranslational Modification Profiling Across Multiple Biological Samples.
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    Chapter 8 Characterization of Individual Histone Posttranslational Modifications and Their Combinatorial Patterns by Mass Spectrometry-Based Proteomics Strategies.
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    Chapter 9 Production and Purification of Antibodies Against Histone Modifications.
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    Chapter 10 Immunofluorescence of Histone Proteins.
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    Chapter 11 Acid-Urea Gel Electrophoresis and Western Blotting of Histones.
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    Chapter 12 Chromatin Immunoprecipitation of Histone Modifications in Fission Yeast.
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    Chapter 13 A Spiking Strategy for ChIP-chip Data Normalization in S. cerevisiae.
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    Chapter 14 High-Resolution Genome-Wide Mapping of Nucleosome Positioning and Occupancy Level Using Paired-End Sequencing Technology.
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    Chapter 15 Physarum polycephalum for Studying the Function of Histone Modifications In Vivo.
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    Chapter 16 A Method for Large-Scale Screening of Random Sequence Libraries to Determine the Function of Unstructured Regions from Essential Proteins.
Attention for Chapter 5: Histone Purification from Saccharomyces cerevisiae.
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Chapter title
Histone Purification from Saccharomyces cerevisiae.
Chapter number 5
Book title
Histones
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-6630-1_5
Pubmed ID
27854016
Book ISBNs
978-1-4939-6628-8, 978-1-4939-6630-1
Authors

Frederic Jourquin, Vincent Géli

Editors

Benoit Guillemette, Luc R. Gaudreau

Abstract

The nucleosome structure consists of a histone octamer made by a tetramer of H3-H4 histones and two dimers of H2A-H2B. Nucleosomes undergo extensive posttranslational modifications that regulate nucleosome interactions, position, and stability.We describe a protocol allowing the robust purification of histones from the yeast Saccharomyces cerevisiae. This method appears to be suitable to quantitatively analyze specific posttranslational histone modifications.

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Mendeley readers

The data shown below were compiled from readership statistics for 12 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 12 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 6 50%
Researcher 4 33%
Professor 1 8%
Unknown 1 8%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 5 42%
Agricultural and Biological Sciences 2 17%
Pharmacology, Toxicology and Pharmaceutical Science 1 8%
Unknown 4 33%

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