| Chapter title |
Production and Purification of Antibodies Against Histone Modifications.
|
|---|---|
| Chapter number | 9 |
| Book title |
Histones
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6630-1_9 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6628-8, 978-1-4939-6630-1
|
| Authors |
Benoit Guillemette, Ian Hammond-Martel, Hugo Wurtele, Alain Verreault |
| Editors |
Benoit Guillemette, Luc R. Gaudreau |
| Abstract |
Antibodies that recognize specific histone modifications are invaluable tools to study chromatin structure and function. There are numerous commercially available antibodies that recognize a remarkable diversity of histone modifications. Unfortunately, many of them fail to work in certain applications or lack the high degree of specificity required of these reagents. The production of affinity-purified polyclonal antibodies against histone modifications demands a little effort but, in return, provides extremely valuable tools that overcome many of the concerns and limitations of commercial antibodies. We present a series of protocols and guidelines for the production and use of large amounts of polyclonal antibodies that recognize modifications of canonical histones. Our protocols can be applied to obtain antibodies that occur in histone variants and proteins other than histones. In addition, some of our protocols are compatible with the production of monoclonal or recombinant antibodies. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 9 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 3 | 33% |
| Student > Master | 2 | 22% |
| Researcher | 1 | 11% |
| Student > Bachelor | 1 | 11% |
| Unknown | 2 | 22% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 4 | 44% |
| Agricultural and Biological Sciences | 3 | 33% |
| Nursing and Health Professions | 1 | 11% |
| Unknown | 1 | 11% |