Chapter title |
Synthetic Protocol for AFCS: A Biologically Active Fluorescent Castasterone Analog Conjugated to an Alexa Fluor 647 Dye
|
---|---|
Chapter number | 2 |
Book title |
Brassinosteroids
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6813-8_2 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6811-4, 978-1-4939-6813-8
|
Authors |
Johan M. Winne, Niloufer G. Irani, Jos Van den Begin, Annemieke Madder |
Editors |
Eugenia Russinova, Ana I. Caño-Delgado |
Abstract |
Synthetic derivatization of hormonally active brassinosteroids (BRs) can provide useful small molecule tools to probe BR signaling pathways, such as fluorescent analogs. However, most biologically active BRs are not suitable for direct chemical conjugation techniques because their derivatization typically requires extensive synthetic work and chemistry expertise. Here, we describe an operationally simple, two-step procedure to prepare and purify an Alexa Fluor 647-castasterone (AFCS) from commercially available materials. The reported strategy is also amenable to the introduction of various other amine-based labeling groups. |
Mendeley readers
Geographical breakdown
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Demographic breakdown
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Researcher | 1 | 25% |
Student > Postgraduate | 1 | 25% |
Student > Master | 1 | 25% |
Readers by discipline | Count | As % |
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Unknown | 1 | 25% |