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Brassinosteroids

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Cover of 'Brassinosteroids'

Table of Contents

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    Book Overview
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    Chapter 1 Protocol for Extraction and Isolation of Brassinosteroids from Plant Tissues
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    Chapter 2 Synthetic Protocol for AFCS: A Biologically Active Fluorescent Castasterone Analog Conjugated to an Alexa Fluor 647 Dye
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    Chapter 3 Physiological Analysis of Brassinosteroid Responses and Sensitivity in Rice
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    Chapter 4 Light Regulation of Brassinosteroid Signaling Components: Checking Regulation of Protein Stability in Darkness
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    Chapter 5 Approaches to Study Light Effects on Brassinosteroid Sensitivity
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    Chapter 6 A Technical Framework for Studying the Signaling Nexus of Brassinosteroids and Immunity
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    Chapter 7 Identification of Brassinosteroid Target Genes by Chromatin Immunoprecipitation Followed by High-Throughput Sequencing (ChIP-seq) and RNA-Sequencing
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    Chapter 8 Quantitation of Cell Type-Specific Responses to Brassinosteroid by Deep Sequencing of Polysome-Associated Polyadenylated RNA
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    Chapter 9 Methods for Modeling Brassinosteroid-Mediated Signaling in Plant Development
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    Chapter 10 Quantitative Microscopic Analysis of Plasma Membrane Receptor Dynamics in Living Plant Cells
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    Chapter 11 Analysis of In Vitro DNA Interactions of Brassinosteroid-Controlled Transcription Factors Using Electrophoretic Mobility Shift Assay
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    Chapter 12 Identification of Brassinosteroid Signaling Complexes by Coimmunoprecipitation and Mass Spectrometry
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    Chapter 13 Simplified Enrichment of Plasma Membrane Proteins from Arabidopsis thaliana Seedlings Using Differential Centrifugation and Brij-58 Treatment
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    Chapter 14 Probing Activation and Deactivation of the BRASSINOSTEROID INSENSITIVE1 Receptor Kinase by Immunoprecipitation
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    Chapter 15 The Primary Root of Sorghum bicolor (L. Moench) as a Model System to Study Brassinosteroid Signaling in Crops
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    Chapter 16 Brassinosteroid Action in Plant Abiotic Stress Tolerance
Attention for Chapter 9: Methods for Modeling Brassinosteroid-Mediated Signaling in Plant Development
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Chapter title
Methods for Modeling Brassinosteroid-Mediated Signaling in Plant Development
Chapter number 9
Book title
Brassinosteroids
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-6813-8_9
Pubmed ID
Book ISBNs
978-1-4939-6811-4, 978-1-4939-6813-8
Authors

David Frigola, Ana I. Caño-Delgado, Marta Ibañes

Editors

Eugenia Russinova, Ana I. Caño-Delgado

Abstract

Mathematical modeling of biological processes is a useful tool to draw conclusions that are contained in the data, but not directly reachable, as well as to make predictions and select the most efficient follow-up experiments. Here we outline a method to model systems of a few proteins that interact transcriptionally and/or posttranscriptionally, by representing the system as Ordinary Differential Equations and to study the model dynamics and stationary states. We exemplify this method by focusing on the regulation by the brassinosteroid (BR) signaling component BRASSINOSTEROID INSENSITIVE1 ETHYL METHYL SULFONATE SUPPRESSOR1 (BES1) of BRAVO, a quiescence-regulating transcription factor expressed in the quiescent cells of Arabidopsis thaliana roots. The method to extract the stationary states and the dynamics is provided as a Mathematica code and requires basic knowledge of the Mathematica software to be executed.

Mendeley readers

The data shown below were compiled from readership statistics for 5 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 5 100%

Demographic breakdown

Readers by professional status Count As %
Professor > Associate Professor 1 20%
Student > Bachelor 1 20%
Researcher 1 20%
Lecturer 1 20%
Student > Master 1 20%
Other 0 0%
Readers by discipline Count As %
Agricultural and Biological Sciences 3 60%
Business, Management and Accounting 1 20%
Biochemistry, Genetics and Molecular Biology 1 20%