Chapter title |
Identification of Brassinosteroid Target Genes by Chromatin Immunoprecipitation Followed by High-Throughput Sequencing (ChIP-seq) and RNA-Sequencing
|
---|---|
Chapter number | 7 |
Book title |
Brassinosteroids
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6813-8_7 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6811-4, 978-1-4939-6813-8
|
Authors |
Trevor Nolan, Sanzhen Liu, Hongqing Guo, Lei Li, Patrick Schnable, Yanhai Yin |
Editors |
Eugenia Russinova, Ana I. Caño-Delgado |
Abstract |
Brassinosteroids (BRs) play important roles in many growth and developmental processes. BRs signal to regulate BR-INSENSITIVE1-ETHYL METHANESULFONATE-SUPPRESSOR1 (BES1) and BRASSINAZOLE-RESISTANT1 (BZR1) transcription factors (TFs), which, in turn, regulate several hundreds of transcription factors (termed BES1/BZR1-targeted TFs or BTFs) and thousands of genes to mediate various BR responses. Chromatin Immunoprecipitation followed by high-throughput sequencing (ChIP-seq) with BES1/BZR1 and BTFs is an important approach to identify BR target genes. In combination with RNA-sequencing experiments, these genomic methods have become powerful tools to detect BR target genes and reveal transcriptional networks underlying BR-regulated processes. |
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