| Chapter title |
Light Regulation of Brassinosteroid Signaling Components: Checking Regulation of Protein Stability in Darkness
|
|---|---|
| Chapter number | 4 |
| Book title |
Brassinosteroids
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6813-8_4 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6811-4, 978-1-4939-6813-8
|
| Authors |
Claudia Corvalán, Sunghwa Choe |
| Editors |
Eugenia Russinova, Ana I. Caño-Delgado |
| Abstract |
Environmental conditions can affect stability of proteins at transcriptional or posttranscriptional levels to modulate their functions. Here we describe a method to observe changes in protein stability under different light conditions. In brief, Arabidopsis thaliana seedlings were maintained under various light regimes from continuous light to total darkness or transitions from light to dark, whereafter total protein was extracted from plants. Proteins were measured and resolved on sodium dodecyl sulfate-polyacrylamide gels and transferred to polyvinylidene difluoride membranes. Blots were incubated with the corresponding antibodies for the visualization of protein bands. The protocol described has been successfully applied in wild-type, different transgenic, and mutant background plants to study how light alone or in combination with other factors influences protein stability. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Professor > Associate Professor | 1 | 100% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 1 | 100% |