| Chapter title |
Synthetic Protein Switches: Theoretical and Experimental Considerations
|
|---|---|
| Chapter number | 1 |
| Book title |
Synthetic Protein Switches
|
| Published in |
Methods in molecular biology, March 2017
|
| DOI | 10.1007/978-1-4939-6940-1_1 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6938-8, 978-1-4939-6940-1
|
| Authors |
Stein, Viktor, Viktor Stein |
| Editors |
Viktor Stein |
| Abstract |
Synthetic protein switches with tailored response functions are finding increasing applications as tools in basic research and biotechnology. With a number of successful design strategies emerging, the construction of synthetic protein switches still frequently necessitates an integrated approach that combines detailed biochemical and biophysical characterization in combination with high-throughput screening to construct tailored synthetic protein switches. This is increasingly complemented by computational strategies that aim to reduce the need for costly empirical optimization and thus facilitate the protein design process. Successful computational design approaches range from analyzing phylogenetic data to infer useful structural, biophysical, and biochemical information to modeling the structure and function of proteins ab initio. The following chapter provides an overview over the theoretical considerations and experimental approaches that have been successful applied in the construction of synthetic protein switches. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| China | 1 | 8% |
| Unknown | 11 | 92% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 4 | 33% |
| Student > Ph. D. Student | 2 | 17% |
| Student > Bachelor | 2 | 17% |
| Student > Master | 1 | 8% |
| Unknown | 3 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 4 | 33% |
| Biochemistry, Genetics and Molecular Biology | 3 | 25% |
| Chemistry | 1 | 8% |
| Unknown | 4 | 33% |