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Synthetic Protein Switches

Overview of attention for book
Cover of 'Synthetic Protein Switches'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Synthetic Protein Switches: Theoretical and Experimental Considerations
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    Chapter 2 Construction of Allosteric Protein Switches by Alternate Frame Folding and Intermolecular Fragment Exchange
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    Chapter 3 Construction of Protein Switches by Domain Insertion and Directed Evolution
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    Chapter 4 Catalytic Amyloid Fibrils That Bind Copper to Activate Oxygen
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    Chapter 5 Ancestral Protein Reconstruction and Circular Permutation for Improving the Stability and Dynamic Range of FRET Sensors
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    Chapter 6 Method for Developing Optical Sensors Using a Synthetic Dye-Fluorescent Protein FRET Pair and Computational Modeling and Assessment
  8. Altmetric Badge
    Chapter 7 Rational Design and Applications of Semisynthetic Modular Biosensors: SNIFITs and LUCIDs
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    Chapter 8 Ultrasensitive Firefly Luminescent Intermediate-Based Protein-Protein Interaction Assay (FlimPIA) Based on the Functional Complementation of Mutant Firefly Luciferases
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    Chapter 9 Quantitative and Dynamic Imaging of ATM Kinase Activity
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    Chapter 10 Creation of Antigen-Dependent β-Lactamase Fusion Protein Tethered by Circularly Permuted Antibody Variable Domains
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    Chapter 11 Protein and Protease Sensing by Allosteric Derepression
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    Chapter 12 DNA-Specific Biosensors Based on Intramolecular β-Lactamase-Inhibitor Complex Formation
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    Chapter 13 Engineering and Characterizing Synthetic Protease Sensors and Switches
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    Chapter 14 Characterizing Dynamic Protein–Protein Interactions Using the Genetically Encoded Split Biosensor Assay Technique Split TEV
  16. Altmetric Badge
    Chapter 15 Development of a Synthetic Switch to Control Protein Stability in Eukaryotic Cells with Light
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    Chapter 16 Light-Regulated Protein Kinases Based on the CRY2-CIB1 System
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    Chapter 17 Yeast-Based Screening System for the Selection of Functional Light-Driven K+ Channels
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    Chapter 18 Primer-Aided Truncation for the Creation of Hybrid Proteins
  20. Altmetric Badge
    Chapter 19 Engineering Small Molecule Responsive Split Protein Kinases
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    Chapter 20 Directed Evolution Methods to Rewire Signaling Networks
Attention for Chapter 17: Yeast-Based Screening System for the Selection of Functional Light-Driven K+ Channels
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  • High Attention Score compared to outputs of the same age and source (84th percentile)

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Chapter title
Yeast-Based Screening System for the Selection of Functional Light-Driven K+ Channels
Chapter number 17
Book title
Synthetic Protein Switches
Published in
Methods in molecular biology, March 2017
DOI 10.1007/978-1-4939-6940-1_17
Pubmed ID
Book ISBNs
978-1-4939-6938-8, 978-1-4939-6940-1
Authors

Cristian Cosentino, Laura Alberio, Gerhard Thiel, Anna Moroni

Editors

Viktor Stein

Abstract

Ion channels control the electrical properties of cells by opening and closing (gating) in response to a wide palette of environmental and physiological stimuli. Endowing ion channels with the possibility to be gated by remotely applied stimuli, such as light, provides a tool for in vivo control of cellular functions in behaving animals. We have engineered a synthetic light-gated potassium (K(+)) channel by connecting an exogenous plant photoreceptor LOV2 domain to the K(+) channel pore Kcv. Here, we describe the experimental strategy that we have used to evolve the properties of the channel toward full control of light on pore gating. Our method combines rational and random mutagenesis of the channel followed by a yeast-based screening system for light-activated K(+) conductance.

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 11 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 11 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 5 45%
Student > Ph. D. Student 2 18%
Student > Bachelor 1 9%
Student > Master 1 9%
Professor 1 9%
Other 1 9%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 4 36%
Agricultural and Biological Sciences 4 36%
Pharmacology, Toxicology and Pharmaceutical Science 1 9%
Neuroscience 1 9%
Unknown 1 9%

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 14 February 2018.
All research outputs
#7,220,889
of 12,510,237 outputs
Outputs from Methods in molecular biology
#2,186
of 8,346 outputs
Outputs of similar age
#143,711
of 290,421 outputs
Outputs of similar age from Methods in molecular biology
#4
of 32 outputs
Altmetric has tracked 12,510,237 research outputs across all sources so far. This one is in the 40th percentile – i.e., 40% of other outputs scored the same or lower than it.
So far Altmetric has tracked 8,346 research outputs from this source. They receive a mean Attention Score of 2.1. This one has gotten more attention than average, scoring higher than 70% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 290,421 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 47th percentile – i.e., 47% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 32 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 84% of its contemporaries.