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Chapter title |
Creation of Antigen-Dependent β-Lactamase Fusion Protein Tethered by Circularly Permuted Antibody Variable Domains
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Chapter number | 10 |
Book title |
Synthetic Protein Switches
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Published in |
Methods in molecular biology, March 2017
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DOI | 10.1007/978-1-4939-6940-1_10 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6938-8, 978-1-4939-6940-1
|
Authors |
Hiroto Iwai, Miki Kojima-Misaizu, Jinhua Dong, Hiroshi Ueda |
Editors |
Viktor Stein |
Abstract |
Antibody-based molecular switches that are able to recognize a range of exogenous antigens can be highly useful as a versatile biosensor. However, regulating the catalytic activity of enzymes by antibodies is still hard to achieve. Here, we describe a design method of unique antibody variable region Fv introduced with two circular permutations, called Clampbody. By tethering the Clampbody to a circularly permuted TEM-1 β-lactamase (BLA), we successfully constructed a genetically encoded molecular switch Cbody-cpBLA that shows antigen-dependent catalytic activity. |
Mendeley readers
The data shown below were compiled from readership statistics for 3 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 3 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Professor | 2 | 67% |
Unspecified | 1 | 33% |
Readers by discipline | Count | As % |
---|---|---|
Unspecified | 1 | 33% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 33% |
Unknown | 1 | 33% |