Chapter title |
ELISA Methods for the Detection of Ebolavirus Infection
|
---|---|
Chapter number | 29 |
Book title |
Ebolaviruses
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-7116-9_29 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7115-2, 978-1-4939-7116-9
|
Authors |
Robert W. Cross, Thomas G. Ksiazek, Cross, Robert W., Ksiazek, Thomas G. |
Editors |
Thomas Hoenen, Allison Groseth |
Abstract |
Ebola viruses are high-priority pathogens first discovered in rural Africa associated with sporadic outbreaks of severe hemorrhagic disease in humans and nonhuman primates. Little is known about the disease ecology or the prevalence of past exposure of human populations to any of the five species of the genus Ebolavirus. The use of immunologic means of detection for either virus antigens or the host's immune response to antigen associated with prior infections offers a powerful approach at understanding the epidemiology and epizootiology of these agents. Here we describe methods for preparing antigen detection sandwich enzyme-linked immunosorbent assays (ELISAs) as well as IgG and IgM ELISAs for the detection of ebolavirus antigens or antibodies in biological samples. |
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Members of the public | 1 | 100% |
Mendeley readers
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Unknown | 19 | 100% |
Demographic breakdown
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Other | 3 | 16% |
Student > Ph. D. Student | 3 | 16% |
Student > Bachelor | 2 | 11% |
Student > Postgraduate | 2 | 11% |
Other | 1 | 5% |
Unknown | 4 | 21% |
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Medicine and Dentistry | 2 | 11% |
Veterinary Science and Veterinary Medicine | 1 | 5% |
Nursing and Health Professions | 1 | 5% |
Other | 1 | 5% |
Unknown | 6 | 32% |