Chapter title |
A Semi-automated High-Throughput Microtitration Assay for Filoviruses
|
---|---|
Chapter number | 12 |
Book title |
Ebolaviruses
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-7116-9_12 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7115-2, 978-1-4939-7116-9
|
Authors |
Claire Marie Filone, David Miller, Victoria Wahl-Jensen |
Editors |
Thomas Hoenen, Allison Groseth |
Abstract |
The 50% tissue culture infectious dose (TCID50) endpoint dilution assay is one of the gold standard methods for measuring filovirus infectivity. We have increased virology microtitration assay throughput at biosafety level (BSL)-4 by implementing automated liquid handling and semi-automated assay endpoint readout. Utilization of automated liquid handling for cell plating and virus dilution along with optimization of the assay endpoint readout, using a luminescent-based cell viability assay and an automated plate reader, has improved workflow efficiency, reduced operator burden and assay time, decreased assay variability, and increased data return. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 2 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Other | 1 | 50% |
Student > Master | 1 | 50% |
Readers by discipline | Count | As % |
---|---|---|
Veterinary Science and Veterinary Medicine | 1 | 50% |
Agricultural and Biological Sciences | 1 | 50% |