Chapter title |
Monitoring Innate Immune Gene Responses in the Hamster Model of Ebola Virus Disease by RT-PCR
|
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Chapter number | 26 |
Book title |
Ebolaviruses
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-7116-9_26 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7115-2, 978-1-4939-7116-9
|
Authors |
Marko Zivcec Ph.D., Marko Zivcec |
Editors |
Thomas Hoenen, Allison Groseth |
Abstract |
Ebola virus (EBOV) disease is a severe, acute human syndrome associated with high case fatality rates. Immune responses to EBOV are thought to be at least partially responsible for disease pathogenesis and must therefore be investigated to get a better understanding of underlying mechanisms of pathogenesis. Syrian hamsters are susceptible to EBOV infection and develop a disease more consistent with human EBOV disease than other rodent disease models. Quantitative RT-PCR (qRT-PCR) is ideal for monitoring immune responses during EBOV infection in low- to medium-throughput applications. A relatively straightforward protocol for monitoring immune responses, based on information gleaned from experimental EBOV infection of hamsters, is presented. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 9 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 5 | 56% |
Other | 2 | 22% |
Student > Master | 1 | 11% |
Unknown | 1 | 11% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 2 | 22% |
Agricultural and Biological Sciences | 2 | 22% |
Veterinary Science and Veterinary Medicine | 1 | 11% |
Medicine and Dentistry | 1 | 11% |
Unknown | 3 | 33% |