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Mendeley readers
| Chapter title |
Spacer-Based Macroarrays for CRISPR Genotyping
|
|---|---|
| Chapter number | 7 |
| Book title |
CRISPR
|
| Published in |
Methods in molecular biology, January 2015
|
| DOI | 10.1007/978-1-4939-2687-9_7 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-2686-2, 978-1-4939-2687-9
|
| Authors |
Igor Mokrousov, Nalin Rastogi |
| Editors |
Magnus Lundgren, Emmanuelle Charpentier, Peter C. Fineran |
| Abstract |
Macroarray-based analysis is a powerful and economic format to study variations in "clustered regularly interspaced short palindromic repeat (CRISPR)" loci in bacteria. To date, it was used almost exclusively for Mycobacterium tuberculosis and was named spoligotyping (spacer oligonucleotides typing). Here, we describe the pipeline of this approach that includes search of loci and selection of spacers, preparation of the membrane with immobilized probes and spoligotyping itself (PCR and reverse hybridization). |
Mendeley readers
The data shown below were compiled from readership statistics for 16 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 16 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 6 | 38% |
| Professor > Associate Professor | 2 | 13% |
| Student > Ph. D. Student | 1 | 6% |
| Other | 1 | 6% |
| Student > Master | 1 | 6% |
| Other | 1 | 6% |
| Unknown | 4 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 3 | 19% |
| Agricultural and Biological Sciences | 3 | 19% |
| Immunology and Microbiology | 2 | 13% |
| Medicine and Dentistry | 2 | 13% |
| Unknown | 6 | 38% |