| Chapter title |
Proteomic Analysis of Lung Tissue by DIGE
|
|---|---|
| Chapter number | 14 |
| Book title |
Difference Gel Electrophoresis
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-7268-5_14 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7267-8, 978-1-4939-7268-5
|
| Authors |
Jarlath E. Nally, Simone Schuller |
| Abstract |
Lungs perform an essential physiological function, mediated by a complex series of events that involve the coordination of multiple cell types to support not only gaseous exchange, but homeostasis and protection from infection. Guinea pigs are an important animal disease model for a number of infectious and noninfectious pulmonary conditions and the availability of a complete genome facilitates comprehensive analysis of tissues using the tools of proteomics. Here, we describe the application of 2-D Difference Gel Electrophoresis (DIGE) to compare, quantify, and identify differential protein expression of proteins in lung tissue from guinea pigs with leptospiral pulmonary hemorrhage syndrome (LPHS) compared to noninfected controls. 2-D DIGE is a powerful technique that provides novel insights into the dynamics of the complex lung proteome during health and disease. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 5 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 2 | 40% |
| Professor | 1 | 20% |
| Student > Master | 1 | 20% |
| Unknown | 1 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Veterinary Science and Veterinary Medicine | 2 | 40% |
| Agricultural and Biological Sciences | 1 | 20% |
| Social Sciences | 1 | 20% |
| Unknown | 1 | 20% |