Nuclear Magnetic Resonance Approaches for Characterizing Protein-Protein Interactions
Methods in molecular biology, January 2018
Yuki Toyama, Yoko Mase, Hanaho Kano, Mariko Yokogawa, Masanori Osawa, Ichio Shimada
The gating of potassium ion (K(+)) channels is regulated by various kinds of protein-protein interactions (PPIs). Structural investigations of these PPIs provide useful information not only for understanding the gating mechanisms of K(+) channels, but also for developing the pharmaceutical compounds targeting K(+) channels. Here, we describe a nuclear magnetic resonance spectroscopic method, termed the cross saturation (CS) method, to accurately determine the binding surfaces of protein complexes, and its application to the investigation of the interaction between a G protein-coupled inwardly rectifying K(+) channel and a G protein α subunit.
|Readers by professional status||Count||As %|
|Student > Ph. D. Student||1||17%|
|Readers by discipline||Count||As %|
|Biochemistry, Genetics and Molecular Biology||3||50%|