Nuclear Magnetic Resonance Approaches for Characterizing Protein-Protein Interactions
Methods in molecular biology, January 2018
Yuki Toyama, Yoko Mase, Hanaho Kano, Mariko Yokogawa, Masanori Osawa, Ichio Shimada
The gating of potassium ion (K(+)) channels is regulated by various kinds of protein-protein interactions (PPIs). Structural investigations of these PPIs provide useful information not only for understanding the gating mechanisms of K(+) channels, but also for developing the pharmaceutical compounds targeting K(+) channels. Here, we describe a nuclear magnetic resonance spectroscopic method, termed the cross saturation (CS) method, to accurately determine the binding surfaces of protein complexes, and its application to the investigation of the interaction between a G protein-coupled inwardly rectifying K(+) channel and a G protein α subunit.
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