| Chapter title |
MOBE-ChIP: Probing Cell Type-Specific Binding Through Large-Scale Chromatin Immunoprecipitation
|
|---|---|
| Chapter number | 15 |
| Book title |
Chromatin Immunoprecipitation
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-7380-4_15 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7379-8, 978-1-4939-7380-4
|
| Authors |
Shenqi Wang, On Sun Lau |
| Abstract |
In multicellular organisms, the initiation and maintenance of specific cell types often require the activity of cell type-specific transcriptional regulators. Understanding their roles in gene regulation is crucial but probing their DNA targets in vivo, especially in a genome-wide manner, remains a technical challenge with their limited expression. To improve the sensitivity of chromatin immunoprecipitation (ChIP) for detecting the cell type-specific signals, we have developed the Maximized Objects for Better Enrichment (MOBE)-ChIP, where ChIP is performed at a substantially larger experimental scale and under low background conditions. Here, we describe the procedure in the study of transcription factors in the model plant Arabidopsis. However, with some modifications, the technique should also be implemented in other systems. Besides cell type-specific studies, MOBE-ChIP can also be used as a general strategy to improve ChIP signals. |
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| Readers by discipline | Count | As % |
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| Biochemistry, Genetics and Molecular Biology | 2 | 100% |