| Chapter title |
Characterization of the Nucleosome Landscape by Micrococcal Nuclease-Sequencing (MNase-seq)
|
|---|---|
| Chapter number | 8 |
| Book title |
Chromatin Immunoprecipitation
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-7380-4_8 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7379-8, 978-1-4939-7380-4
|
| Authors |
Wieteke Anna Maria Hoeijmakers, Richárd Bártfai, Hoeijmakers, Wieteke Anna Maria, Bártfai, Richárd |
| Abstract |
MNase-seq allows the genome-wide examination of the nucleosome landscape by determination of nucleosome positioning and occupancy. Typically, native or formaldehyde fixed chromatin is subjected to digestion by micrococcal nuclease (MNase), which degrades linker DNA and yields mainly mono-nucleosomes. The resulting material can be processed directly or can be subjected to an optional chromatin immunoprecipitation step (MNase-ChIP-seq). De-crosslinked and purified DNA is then subjected to next-generation sequencing. The protocol presented here has been tailored for the analysis of nucleosome landscape in the malaria parasite, Plasmodium falciparum, but most steps are directly applicable to other cell types. We also discuss general considerations for experimental design and computational analysis, which are crucial for accurate investigation of the nucleosome landscape. |
X Demographics
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|---|---|---|
| Unknown | 2 | 100% |
Demographic breakdown
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|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
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| Country | Count | As % |
|---|---|---|
| Unknown | 22 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 8 | 36% |
| Researcher | 4 | 18% |
| Student > Bachelor | 3 | 14% |
| Student > Master | 3 | 14% |
| Unspecified | 1 | 5% |
| Other | 1 | 5% |
| Unknown | 2 | 9% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 8 | 36% |
| Agricultural and Biological Sciences | 5 | 23% |
| Medicine and Dentistry | 2 | 9% |
| Unspecified | 1 | 5% |
| Immunology and Microbiology | 1 | 5% |
| Other | 1 | 5% |
| Unknown | 4 | 18% |