Chapter title |
Quantification of Bacterial Chemotaxis Responses at the Mouths of Hydrogel Capillaries
|
---|---|
Chapter number | 4 |
Book title |
Bacterial Chemosensing
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7577-8_4 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7576-1, 978-1-4939-7577-8
|
Authors |
Benjamin A. Webb, Timofey D. Arapov, Birgit E. Scharf |
Abstract |
Many chemotaxis assays allow for the assessment of bacterial chemotaxis by determining the number of cells migrating toward a chemoattractant or away from a chemorepellent. Some of these assays use a capillary filled with a chemoeffector/agarose mixture to allow cells to accumulate at the mouth of the capillary. Subsequently, assumptions about the relative strengths of chemotaxis strength are based on visual comparisons. Here, we describe a modification of this assay that uses a hydrogel matrix to enable quantitative time-course measurements by analyzing image pixel intensities. This approach allows a high-throughput method when coupled with the aid of a motorized microscope stage. |
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