Chapter title |
Monitoring Two-Component Sensor Kinases with a Chemotaxis Signal Readout
|
---|---|
Chapter number | 12 |
Book title |
Bacterial Chemosensing
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7577-8_12 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7576-1, 978-1-4939-7577-8
|
Authors |
Run-Zhi Lai, John S. Parkinson |
Abstract |
Bacteria use two-component signal transduction systems to elicit adaptive responses to environmental changes. The simplest of these systems comprises a transmembrane sensor with histidine kinase activity and its cytoplasmic response regulator partner. Stimulus-response studies of two-component signaling systems typically employ expression reporters, such as β-galactosidase, that operate with relatively slow kinetics and low precision. In this chapter, we illustrate a new strategy for directly measuring the signaling activities of two-component sensor kinases in vivo. Our method exploits recent work that defines the recognition determinants for sensor-response regulator signaling transactions, which enabled us to couple histidine kinases to a FRET-based assay that uses signaling components of the E. coli chemotaxis system. We demonstrate the approach with NarX, a nitrate/nitrite sensor kinase, but the method should be applicable to other two-component sensor kinases. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 5 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Unspecified | 1 | 20% |
Librarian | 1 | 20% |
Student > Ph. D. Student | 1 | 20% |
Unknown | 2 | 40% |
Readers by discipline | Count | As % |
---|---|---|
Unspecified | 1 | 20% |
Biochemistry, Genetics and Molecular Biology | 1 | 20% |
Computer Science | 1 | 20% |
Unknown | 2 | 40% |