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Bacterial Chemosensing

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Cover of 'Bacterial Chemosensing'

Table of Contents

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    Book Overview
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    Chapter 1 The Diversity of Bacterial Chemosensing
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    Chapter 2 Transmembrane Signal Transduction in Bacterial Chemosensing
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    Chapter 3 Two Spatial Chemotaxis Assays: The Nutrient-Depleted Chemotaxis Assay and the Agarose-Plug-Bridge Assay
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    Chapter 4 Quantification of Bacterial Chemotaxis Responses at the Mouths of Hydrogel Capillaries
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    Chapter 5 A Static Microfluidic Device for Investigating the Chemotaxis Response to Stable, Non-linear Gradients
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    Chapter 6 Visualizing Chemoattraction of Planktonic Cells to a Biofilm
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    Chapter 7 Labeling Bacterial Flagella with Fluorescent Dyes
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    Chapter 8 All-Codon Mutagenesis for Structure-Function Studies of Chemotaxis Signaling Proteins
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    Chapter 9 Mutational Analysis of Binding Protein–Chemoreceptor Interactions
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    Chapter 10 In Vitro Assay for Measuring Receptor-Kinase Activity in the Bacillus subtilis Chemotaxis Pathway
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    Chapter 11 FRET Analysis of the Chemotaxis Pathway Response
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    Chapter 12 Monitoring Two-Component Sensor Kinases with a Chemotaxis Signal Readout
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    Chapter 13 Analyzing Protein Domain Interactions in Chemoreceptors by In Vivo PEGylation
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    Chapter 14 Tuning Chemoreceptor Signaling by Positioning Aromatic Residues at the Lipid–Aqueous Interface
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    Chapter 15 Analyzing Chemoreceptor Interactions In Vivo with the Trifunctional Cross-Linker TMEA
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    Chapter 16 Use of Cryo-EM to Study the Structure of Chemoreceptor Arrays In Vivo
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    Chapter 17 Visualizing Chemoreceptor Arrays in Bacterial Minicells by Cryo-Electron Tomography and Subtomogram Analysis
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    Chapter 18 Bacterial Chemoreceptor Imaging at High Spatiotemporal Resolution Using Photoconvertible Fluorescent Proteins
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    Chapter 19 Imaging of Single Dye-Labeled Chemotaxis Proteins in Live Bacteria Using Electroporation
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    Chapter 20 Fluorescence Anisotropy to Detect In Vivo Stimulus-Induced Changes in Chemoreceptor Packing
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    Chapter 21 Chemotaxis to Atypical Chemoattractants by Soil Bacteria
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    Chapter 22 Screening Chemoreceptor–Ligand Interactions by High-Throughput Thermal-Shift Assays
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    Chapter 23 High-Throughput Screening to Identify Chemoreceptor Ligands
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    Chapter 24 Identification of Specific Ligands for Sensory Receptors by Small-Molecule Ligand Arrays and Surface Plasmon Resonance
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    Chapter 25 Fluorescence Measurement of Kinetics of CheY Autophosphorylation with Small Molecule Phosphodonors
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    Chapter 26 Synthesis of a Stable Analog of the Phosphorylated Form of CheY: Phosphono-CheY
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    Chapter 27 Quantitative Modeling of Flagellar Motor-Mediated Adaptation
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    Chapter 28 Molecular Modeling of Chemoreceptor:Ligand Interactions
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    Chapter 29 Phylogenetic and Protein Sequence Analysis of Bacterial Chemoreceptors
Attention for Chapter 9: Mutational Analysis of Binding Protein–Chemoreceptor Interactions
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Chapter title
Mutational Analysis of Binding Protein–Chemoreceptor Interactions
Chapter number 9
Book title
Bacterial Chemosensing
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7577-8_9
Pubmed ID
Book ISBNs
978-1-4939-7576-1, 978-1-4939-7577-8
Authors

Andrew L. Seely

Abstract

Interactions between ligands and chemoreceptors in Escherichia coli and Salmonella enterica can be studied through genetic manipulation of the actors involved. Sequence analysis and modeling can reveal potential sites of interaction, and these sites can be deleted or mutated and the effects tested through various in vivo chemotaxis assays to ascertain their importance during interaction. Here, the approach for analysis of the interaction between a major E. coli chemoreceptor and its binding protein ligand is described.

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Mendeley readers

The data shown below were compiled from readership statistics for 1 Mendeley reader of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 1 100%

Demographic breakdown

Readers by professional status Count As %
Professor 1 100%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 1 100%