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Chapter title |
Identification of Specific Ligands for Sensory Receptors by Small-Molecule Ligand Arrays and Surface Plasmon Resonance
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Chapter number | 24 |
Book title |
Bacterial Chemosensing
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Published in |
Methods in molecular biology, January 2018
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DOI | 10.1007/978-1-4939-7577-8_24 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7576-1, 978-1-4939-7577-8
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Authors |
Christopher J. Day, Victoria Korolik |
Abstract |
Ligand-receptor interactions triggering signal transduction components of many sensory pathways, remain elusive due to paucity of high-throughput screening methods. Here we describe our use of small molecule microarrays comprising of small glycans, amino and organic acids, salts, and other known chemoeffectors, for screening of ligands specific to various sensory receptors, followed by surface plasmon resonance to verify the veracity and to determine the affinity constants of the interactions. This methodology allows for rapid and identification of the direct ligand binding between the sensory receptors and their specific ligands. |
Mendeley readers
The data shown below were compiled from readership statistics for 4 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 4 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 2 | 50% |
Unspecified | 1 | 25% |
Lecturer | 1 | 25% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 2 | 50% |
Unspecified | 1 | 25% |
Chemical Engineering | 1 | 25% |