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Chapter title |
Visualizing Chemoreceptor Arrays in Bacterial Minicells by Cryo-Electron Tomography and Subtomogram Analysis
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Chapter number | 17 |
Book title |
Bacterial Chemosensing
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Published in |
Methods in molecular biology, January 2018
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DOI | 10.1007/978-1-4939-7577-8_17 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7576-1, 978-1-4939-7577-8
|
Authors |
Zhuan Qin, Bo Hu, Jun Liu |
Abstract |
Bacterial chemoreceptors form a highly ordered array in concert with the CheA kinase and the CheW coupling protein. The precise architecture of the array is responsible for high sensitivity, high dynamic range, and strong amplification of chemotaxis signaling. Cryo-electron tomography (cryo-ET) has emerged as a unique tool to visualize bacterial chemotaxis arrays at molecular level. Here we describe a detailed cryo-ET and subtomogram averaging procedure to determine in situ structure of the chemoreceptor arrays in Salmonella minicells. The procedure should be readily applicable to visualize other large macromolecular assemblies in their cellular context. |
Mendeley readers
The data shown below were compiled from readership statistics for 11 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 11 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 3 | 27% |
Student > Bachelor | 2 | 18% |
Professor | 1 | 9% |
Other | 1 | 9% |
Unknown | 4 | 36% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 3 | 27% |
Agricultural and Biological Sciences | 3 | 27% |
Medicine and Dentistry | 1 | 9% |
Unknown | 4 | 36% |