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Sertoli Cells

Overview of attention for book
Cover of 'Sertoli Cells'

Table of Contents

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    Book Overview
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    Chapter 1 Establishment of Primary Culture of Sertoli Cells
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    Chapter 2 Evaluation of the Purity of Sertoli Cell Primary Cultures
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    Chapter 3 Preparation of Testicular Samples for Histology and Immunohistochemistry
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    Chapter 4 Rabbit Sertoli Cells: Immunohistochemical Profile from Neonatal to Adult Age
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    Chapter 5 Identification of Proliferative and Apoptotic Sertoli Cells Using Fluorescence and Confocal Microscopy
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    Chapter 6 Sertoli Cell Preparation for Co-immunoprecipitation
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    Chapter 7 Profiling Signaling Proteins in Sertoli Cells by Co-immunoprecipitation
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    Chapter 8 Phagocytosis by Sertoli Cells: Analysis of Main Phagocytosis Steps by Confocal and Electron Microscopy
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    Chapter 9 A Method for In Vivo Induction and Ultrastructural Detection of Mitophagy in Sertoli Cells
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    Chapter 10 Assessing Autophagy in Sertoli Cells
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    Chapter 11 Molecular Mechanisms and Signaling Pathways Involved in the Nutritional Support of Spermatogenesis by Sertoli Cells
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    Chapter 12 Assessing Sertoli Cell Metabolic Activity
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    Chapter 13 Proteome Profiling of Sertoli Cells Using a GeLC-MS/MS Strategy
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    Chapter 14 Gene Silencing of Human Sertoli Cells Utilizing Small Interfering RNAs
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    Chapter 15 Testicular Cell Selective Ablation Using Diphtheria Toxin Receptor Transgenic Mice
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    Chapter 16 Regulation of Blood-Testis Barrier (BTB) Dynamics, Role of Actin-, and Microtubule-Based Cytoskeletons
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    Chapter 17 Monitoring the Integrity of the Blood-Testis Barrier (BTB): An In Vivo Assay
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    Chapter 18 Computational Methods Involved in Evaluating the Toxicity of the Reproductive Toxicants in Sertoli Cell
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    Chapter 19 A Stopped-Flow Light Scattering Methodology for Assessing the Osmotic Water Permeability of Whole Sertoli Cells
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    Chapter 20 Cryopreservation of Human Testicular Tissue by Isopropyl-Controlled Slow Freezing
Attention for Chapter 9: A Method for In Vivo Induction and Ultrastructural Detection of Mitophagy in Sertoli Cells
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Chapter title
A Method for In Vivo Induction and Ultrastructural Detection of Mitophagy in Sertoli Cells
Chapter number 9
Book title
Sertoli Cells
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7698-0_9
Pubmed ID
29453568
Book ISBNs
978-1-4939-7697-3, 978-1-4939-7698-0
Authors

Nabil Eid, Yuko Ito, Akio Horibe, Hitomi Hamaoka, Yoichi Kondo, Eid, Nabil, Ito, Yuko, Horibe, Akio, Hamaoka, Hitomi, Kondo, Yoichi

Abstract

An emerging body of evidences based on in vitro studies indicate that mitophagy (selective autophagic clearance of damaged mitochondria) is a prosurvival mechanism, specifically under exposure to various stressors. Sertoli cells (SCs) play essential roles in maintenance of spermatogenesis via paracrine interactions with germ cells and other somatic cells in the testis; however, studies investigating mitophagy in SCs are still very few. In this chapter, we give a brief review of mechanisms and detection methods of mitophagy in SCs based on our recent publications on animal models of ethanol toxicity and current literature. In addition, we provide a method for induction and ultrastructural identification of mitophagy in SCs of adult Wistar rats using a single intraperitoneal injection (5 g/kg) of ethanol. Proper understanding of mitophagy features and mechanisms in SCs may have therapeutic implications for infertility associated with alcoholism and other diseases characterized by mitochondrial dysfunction.

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X Demographics

X Demographics

The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 5 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 5 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 1 20%
Other 1 20%
Student > Master 1 20%
Unknown 2 40%
Readers by discipline Count As %
Medicine and Dentistry 3 60%
Nursing and Health Professions 1 20%
Unknown 1 20%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 February 2018.
All research outputs
#18,587,406
of 23,023,224 outputs
Outputs from Methods in molecular biology
#7,965
of 13,166 outputs
Outputs of similar age
#330,565
of 442,364 outputs
Outputs of similar age from Methods in molecular biology
#950
of 1,499 outputs
Altmetric has tracked 23,023,224 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,166 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 24th percentile – i.e., 24% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 442,364 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 14th percentile – i.e., 14% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 1,499 others from the same source and published within six weeks on either side of this one. This one is in the 20th percentile – i.e., 20% of its contemporaries scored the same or lower than it.

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